Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 28, Pages 5679-5685Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b01531
Keywords
galacto-oligosaccharides; Kluyveromyces lactis; permeabilized cells; purification; recyclable process
Funding
- Ministry of Science and Technology of China [2012YQ090194, 2013AA102204]
- Natural Science Foundation of China [21476165, 21206113]
- Beiyang Young Scholar of Tianjin University
- Program of Introducing Talents of Discipline to Universities of China [B06006]
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A recyclable strategy for the production of high-purity (>95%) galacto-oligosaccharides (GOS) was developed using Kluyveromyces lactis in both the synthesis and purification steps. For the synthesis of GOS, ethanol-permeabilized cells (p cells) of K lactis were used because the enhanced permeability facilitated the mass transfer of the substrate and the release of oligosaccharide products. For the purification of GOS, non-permeabilized K. lactis cells (np-cells) were preferred as a result of their intrinsic cell membrane barrier toward GOS, which led to the selective consumption of carbohydrate. In this way, undesired glucose, galactose, and lactose in the raw GOS solution can be completely removed. This strategy is recyclable not only because of the high stability and reusability of p-cells and np-cells but also because the ethanol, which is simultaneously generated during the purification, can be reused for the preparation of p-cells. The strategy proposed in this study is a promising candidate for the efficient production of high-purity GOS.
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