Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 12, Pages 2523-2530Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b04697
Keywords
hairy roots; isoprenoids; MEP pathway; Salvia miltiorrhiza; tanshinbne
Funding
- Chinese National Natural Science Fund [81522049, 31270007, 30900110, 31571735, 31470407]
- Program for New Century Excellent Talents in University [NECT-13-0902]
- Shanghai Science and Technology Committee Key Project [15430502700, 10JC1412000]
- Fok Ying Tong Education Foundation [131041]
- State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF02]
- Shanghai Education Committee Fund [13ZZ104, J50401]
- Zhejiang Provincial Science and Technology Project [2014C32090, Y2080621]
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Tanshinones are health-promoting diterpenoids found in Salvia miltiorrhiza and have wide applications. Here, SmGGPPS (geranylgeranyl diphosphate synthase) and SmDXSII (1-deoxy-ri-xylulose-5-phosphate synthase) were introduced into hairy roots of S. miltiorrhiza. Overexpression of SmGGPPS and SmDXSII in hairy roots produces higher levels of tanshinone than control and single-gene transformed lines; tanshinone production in the double-gene transformed line GDII10 reached 12.93 mg/g dry weight, which is the highest tanshinone content that has been achieved through genetic engineering. Furthermore, transgenic hairy root lines showed higher antioxidant and antitumor activities than control lines. In addition, contents of chlorophylls, carotenoids, indoleacetic acid, and gibberellins were significantly elevated in transgenic Arabidopsis thaliana plants. These results demonstrate a promising method to improve the production of diterpenoids including tanshinone as well as other natural plastid-derived isoprenoids in plants by genetic manipulation of the 2-Ctrnethyl,p-erythritoI-4-phosphate (MEP) pathway.
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