☆ 4.7 Review

A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Inhibitor-assisted synthesis of molecularly imprinted microbeads for protein recognition

Hasan Basan et al.

ANALYTICAL METHODS (2018)

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Article Biotechnology & Applied Microbiology

Poly(carboxybetaine methacrylate)-grafted silica nanoparticle: A novel carrier for enzyme immobilization

Liqian Zhang et al.

BIOCHEMICAL ENGINEERING JOURNAL (2018)

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Article Biochemistry & Molecular Biology

Rational Engineering of Multiple Active Sites in an Ester Hydrolase

Gerard Santiago et al.

BIOCHEMISTRY (2018)

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Review Biotechnology & Applied Microbiology

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

Claudia Bernal et al.

BIOTECHNOLOGY ADVANCES (2018)

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Review Food Science & Technology

Biotechnological Applications of Proteases in Food Technology

Olga Luisa Tavano et al.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2018)

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Article Biotechnology & Applied Microbiology

Stabilization of dimeric β-glucosidase from Aspergillus niger via glutaraldehyde immobilization under different conditions

Perla Guadalupe Vazquez-Ortega et al.

ENZYME AND MICROBIAL TECHNOLOGY (2018)

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Article Chemistry, Applied

Revisiting the enzymatic kinetics of pepsin using isothermal titration calorimetry

Qi Luo et al.

FOOD CHEMISTRY (2018)

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Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms

Huiru Zhang et al.

LANGMUIR (2018)

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Article Biochemistry & Molecular Biology

Computational redesign of enzymes for regio- and enantioselective hydroamination

Ruifeng Li et al.

NATURE CHEMICAL BIOLOGY (2018)

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Directed Evolution of Protein Catalysts

Cathleen Zeymer et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87 (2018)

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Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

El-Hocine Siar et al.

CATALYSTS (2018)

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Article Allergy

Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure

Jaap Akkerdaas et al.

CLINICAL AND TRANSLATIONAL ALLERGY (2018)

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Selective Binding of Inhibitor-Assisted Surface-Imprinted Core/Shell Microbeads in Protein Mixtures

Mehmet Dinc et al.

CHEMISTRYSELECT (2018)

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Immobilized glucose oxidase on magnetic silica and alumina: Beyond magnetic separation

Rigel Jaquish et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

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Directed Evolution: Bringing New Chemistry to Life

Frances H. Arnold

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

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Review Gastroenterology & Hepatology

Pepsin and Its Importance for Functional Dyspepsia: Relic, Regulator or Remedy?

Karl-Uwe Petersen

DIGESTIVE DISEASES (2018)

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Article Allergy

Reduction of the allergenicity of cow's milk α-lactalbumin under heat-treatment and enzymatic hydrolysis in Moroccan population

O. Azdad et al.

EUROPEAN ANNALS OF ALLERGY AND CLINICAL IMMUNOLOGY (2018)

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Article Biochemistry & Molecular Biology

Determinants and Prediction of Esterase Substrate Promiscuity Patterns

Monica Martinez-Martinez et al.

ACS CHEMICAL BIOLOGY (2018)

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Article Food Science & Technology

The effect of treatments on the allergenicity of β-lactoglobulin in Moroccan population

Ouarda Azdad et al.

NUTRITION & FOOD SCIENCE (2018)

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Role of Biocatalysis in Sustainable Chemistry

Roger A. Sheldon et al.

CHEMICAL REVIEWS (2018)

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Online coupling of immunoextraction, digestion, and microliquid chromatography-tandem mass spectrometry for the analysis of sarin and soman-butyrylcholinesterase adducts in human plasma

Maud Bonichon et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2018)

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Impact of substrate diffusion and enzyme distribution in 3D-porous electrodes: a combined electrochemical and modelling study of a thermostable H2/O2 enzymatic fuel cell

Ievgen Mazurenko et al.

ENERGY & ENVIRONMENTAL SCIENCE (2017)

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Article Biotechnology & Applied Microbiology

Effect of protein load on stability of immobilized enzymes

Laura Fernandez-Lopez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2017)

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Article Chemistry, Multidisciplinary

The E factor 25 years on: the rise of green chemistry and sustainability

Roger A. Sheldon

GREEN CHEMISTRY (2017)

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Article Biochemical Research Methods

Development of immunosorbents coupled on-line to immobilized pepsin reactor and micro liquid chromatography-tandem mass spectrometry for analysis of butyrylcholinesterase in human plasma

Maud Bonichon et al.

JOURNAL OF CHROMATOGRAPHY A (2017)

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Article Biochemistry & Molecular Biology

Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins

El-Hocine Siar et al.

PROCESS BIOCHEMISTRY (2017)

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Article Biochemistry & Molecular Biology

Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads

Hadjer Zaak et al.

PROCESS BIOCHEMISTRY (2017)

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Article Biochemical Research Methods

OpenMM 7: Rapid development of high performance algorithms for molecular dynamics

Peter Eastman et al.

PLOS COMPUTATIONAL BIOLOGY (2017)

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Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β-galactosidase from Aspergillus oryzae

Hadjer Zaak et al.

CATALYSTS (2017)

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Review Chemistry, Physical

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Viridiana Santana Ferreira-Leitao et al.

CATALYSTS (2017)

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Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design

Jennifer Cassidy et al.

PLOS ONE (2017)

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New platform for simple and rapid protein-based affinity reactions

Kei Kubota et al.

SCIENTIFIC REPORTS (2017)

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Article Gastroenterology & Hepatology

A non-interventional, observational study of a fixed combination of pepsin and amino acid hydrochloride in patients with functional dyspepsia

Kristin Forssmann et al.

BMC GASTROENTEROLOGY (2017)

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Article Allergy

Hydrolysed ovalbumin offers more effective preventive and therapeutic protection against egg allergy than the intact protein

D. Lozano-Ojalvo et al.

CLINICAL AND EXPERIMENTAL ALLERGY (2017)

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Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

Alexander Jonsson et al.

ANALYTICAL CHEMISTRY (2017)

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Article Biochemical Research Methods

Gold nanoparticle-conjugated pepsin for efficient solution-like heterogeneous biocatalysis in analytical sample preparation protocols

Markus Hoeldrich et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2016)

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NOVEL APPLICATION OF POROUS AND CELLULAR MATERIALS FOR COVALENT IMMOBILIZATION OF PEPSIN

K. Szalapata et al.

BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING (2016)

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Chemical amination of lipases improves their immobilization on octyl-glyoxyl agarose beads

Nazzoly Rueda et al.

CATALYSIS TODAY (2016)

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Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Juan M. Bolivar et al.

CATALYSIS TODAY (2016)

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Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Nazzoly Rueda et al.

CHEMICAL RECORD (2016)

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Development of immobilized-pepsin microreactors coupled to nano liquid chromatography and tandem mass spectrometry for the quantitative analysis of human butyrylcholinesterase

Maud Bonichon et al.

JOURNAL OF CHROMATOGRAPHY A (2016)

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Preparation of graphene oxide-modified affinity capillary monoliths based on three types of amino donor for chiral separation and proteolysis

Tingting Hong et al.

JOURNAL OF CHROMATOGRAPHY A (2016)

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Article Materials Science, Biomaterials

Inhibitor-assisted synthesis of silica-core microbeads with pepsin-imprinted nanoshells

M. Dinc et al.

JOURNAL OF MATERIALS CHEMISTRY B (2016)

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Article Chemistry, Multidisciplinary

Bacterial keratinolytic protease, imminent starter for NextGen leather and detergent industries

Tanmay Paul et al.

SUSTAINABLE CHEMISTRY AND PHARMACY (2016)

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Review Chemistry, Multidisciplinary

Nanomaterials for biocatalyst immobilization - state of the art and future trends

Eliane P. Cipolatti et al.

RSC ADVANCES (2016)

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Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts

Oveimar Barbosa et al.

BIOTECHNOLOGY ADVANCES (2015)

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Importance of the Support Properties for Immobilization or Purification of Enzymes

Jose Cleiton S. dos Santos et al.

CHEMCATCHEM (2015)

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Advances in Chemical Protein Modification

Omar Boutureira et al.

CHEMICAL REVIEWS (2015)

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Article Biotechnology & Applied Microbiology

Pepsin immobilization on an aldehyde-modified polymethacrylate monolith and its application for protein analysis

Wenjuan Han et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2015)

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Article Biochemistry & Molecular Biology

Immobilization of Pepsin on Chitosan Magnetic Nanoparticles and Its Application in Deacetylation of Amides

Moslem Mansour Lakouraj et al.

JOURNAL OF CARBOHYDRATE CHEMISTRY (2015)

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Article Biochemistry & Molecular Biology

Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachment

Jose C. S. dos Santos et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2015)

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Advances in protease engineering for laundry detergents

Ljubica Vojcic et al.

NEW BIOTECHNOLOGY (2015)

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Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

Jose C. S. dos Santos et al.

RSC ADVANCES (2015)

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Article Polymer Science

The Surface Characteristics of Chitosan Modified PSt-GMA Microspheres Influenced the Interactions and Properties of Immobilized Pepsin

Gang Peng et al.

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY (2015)

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Article Biochemical Research Methods

Immobilized Pepsin Microreactor for Rapid Peptide Mapping with Nanoelectrospray Ionization Mass Spectrometry

Ying Long et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2015)

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Review Chemistry, Physical

Enzyme immobilization by adsorption: a review

Teofil Jesionowski et al.

ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY (2014)

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Enhanced activity of immobilized pepsin nanoparticles coated on solid substrates compared to free pepsin

David Meridor et al.

ENZYME AND MICROBIAL TECHNOLOGY (2014)

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Enzyme immobilization on smart polymers: Catalysis on demand

Giuseppe Cirillo et al.

REACTIVE & FUNCTIONAL POLYMERS (2014)

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Preparation, Characterization, and Luminescence of (SBA-15) Immobilized Pepsin

Qing-Zhou Zhai et al.

RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A (2014)

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Review Multidisciplinary Sciences

Selective chemical protein modification

Christopher D. Spicer et al.

NATURE COMMUNICATIONS (2014)

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Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

Oveimar Barbosa et al.

RSC ADVANCES (2014)

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Review Chemistry, Multidisciplinary

Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Rafael C. Rodrigues et al.

RSC ADVANCES (2014)

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Article Engineering, Chemical

Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Sari Edi Cahyaningrum et al.

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS (2014)

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Article Chemistry, Multidisciplinary

Computational Enzyme Design

Gert Kiss et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)

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Poly(ethyleneimine) cross-linked multilayers deposited onto solid surfaces and enzyme immobilization as a function of the film properties

Florin Bucatariu et al.

APPLIED SURFACE SCIENCE (2013)

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Review Biochemistry & Molecular Biology

Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

Oveimar Barbosa et al.

BIOMACROMOLECULES (2013)

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Review Chemistry, Multidisciplinary

Enzyme immobilisation in biocatalysis: why, what and how

Roger A. Sheldon et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Industrial use of immobilized enzymes

Robert DiCosimo et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods

Sara Cantone et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Modifying enzyme activity and selectivity by immobilization

Rafael C. Rodrigues et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Evaluation of immobilized enzymes for industrial applications

Andreas Liese et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Conformational changes of enzymes upon immobilisation

Francesco Secundo

CHEMICAL SOCIETY REVIEWS (2013)

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Protein hydrolysis using proteases: An important tool for food biotechnology

Olga Luisa Tavano

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2013)

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Article Nanoscience & Nanotechnology

Non-denaturing low-temperature bonding of patterned poly(methyl methacrylate) enzymatic microreactors

Michelle D. Douma et al.

MICROFLUIDICS AND NANOFLUIDICS (2013)

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Prediction and experimental validation of enzyme substrate specificity in protein structures

Shivas R. Amin et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

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Article Pharmacology & Pharmacy

Relationship between protein digestibility and allergenicity: Comparisons of pepsin and cathepsin

Emily S. Foster et al.

TOXICOLOGY (2013)

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Self-Assembly of Synthetic Metabolons through Synthetic Protein Scaffolds: One-Step Purification, Co-immobilization, and Substrate Channeling

Chun You et al.

ACS SYNTHETIC BIOLOGY (2013)

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Article Chemistry, Analytical

Pepsin Immobilized on High-Strength Hybrid Particles for Continuous Flow Online Digestion at 10 000 psi

Joomi Ahn et al.

ANALYTICAL CHEMISTRY (2012)

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Article Materials Science, Ceramics

Active Biocatalysts Based on Pepsin Immobilized in Short Channeled Zr-Ce-SBA-15

Wang Fang et al.

JOURNAL OF INORGANIC MATERIALS (2012)

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Article Polymer Science

Novel synthetic route for covalent coupling of biomolecules on super-paramagnetic hybrid nanoparticles

Antonio L. Medina-Castillo et al.

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY (2012)

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Article Biochemistry & Molecular Biology

Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica

Oveimar Barbosa et al.

PROCESS BIOCHEMISTRY (2012)

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Article Biochemistry & Molecular Biology

The slow-down of the CALB immobilization rate permits to control the inter and intra molecular modification produced by glutaraldehyde

Oveimar Barbosa et al.

PROCESS BIOCHEMISTRY (2012)

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Article Biochemical Research Methods

Digital microfluidic hydrogel microreactors for proteomics

Vivienne N. Luk et al.

PROTEOMICS (2012)

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Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

Rafael C. Rodrigues et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

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Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

Cristina Garcia-Galan et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

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Metagenomic Analyses: Past and Future Trends

Carola Simon et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2011)

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Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

Karel Hernandez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

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Review Biotechnology & Applied Microbiology

Biotransformations in microstructured reactors: more than flowing with the stream?

Juan M. Bolivar et al.

TRENDS IN BIOTECHNOLOGY (2011)

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Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis

Junfeng Ma et al.

ANALYTICAL CHEMISTRY (2010)

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Enzyme technology for precision functional food ingredient processes

Anne S. Meyer

FOODS FOR HEALTH IN THE 21ST CENTURY: A ROAD MAP FOR THE FUTURE (2010)

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Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

G. Volpato et al.

CURRENT MEDICINAL CHEMISTRY (2010)

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Metagenomic era for biocatalyst identification

Lucia Fernandez-Arrojo et al.

CURRENT OPINION IN BIOTECHNOLOGY (2010)

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Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography-mass spectrometry

A. Cingoez et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2010)

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Article Chemistry, Physical

Vinyl sulfone functionalized silica: a ready to use'' pre-activated material for immobilization of biomolecules

Mariano Ortega-Munoz et al.

JOURNAL OF MATERIALS CHEMISTRY (2010)

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Article Biochemistry & Molecular Biology

Immobilization of Geobacillus pallidus RAPc8 nitrile hydratase (NHase) reduces substrate inhibition and enhances thermostability

Idan Chiyanzu et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2010)

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Enzymatic Catalyzed Synthesis and Triggered Gelation of Ionic Peptides

Jean-Baptiste Guilbaud et al.

LANGMUIR (2010)

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Article Chemistry, Organic

Vinyl sulfone: a versatile function for simple bioconjugation and immobilization

Julia Morales-Sanfrutos et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2010)

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Article Biochemistry & Molecular Biology

Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports

Juan M. Bolivar et al.

PROCESS BIOCHEMISTRY (2010)

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Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology

Justin M. Chalker et al.

CHEMISTRY-AN ASIAN JOURNAL (2009)

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Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

Roberto Fernandez-Lafuente

ENZYME AND MICROBIAL TECHNOLOGY (2009)

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HYDROPHILIC DIAZIRINE POLYMER FOR ONE-STEP PHOTO-FABRICATION OF PROTEINS ON POLYPROPYLENE SURFACE

Takenori Tomohiro et al.

HETEROCYCLES (2009)

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Peptide inhibitor modified magnetic particles for pepsin separation

Michaela Filuszova et al.

JOURNAL OF SEPARATION SCIENCE (2009)

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Hydrophobic mesoporous materials for immobilization of enzymes

Mina Park et al.

MICROPOROUS AND MESOPOROUS MATERIALS (2009)

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Article Biochemistry & Molecular Biology

Immobilization-stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

Rafael C. Rodrigues et al.

PROCESS BIOCHEMISTRY (2009)

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Article Chemistry, Analytical

Pepsin immobilized in dextran-modified fused-silica capillaries for on-line protein digestion and peptide mapping

E. C. A. Stigter et al.

ANALYTICA CHIMICA ACTA (2008)

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Article Biochemistry & Molecular Biology

Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose

Gloria Fernandez-Lorente et al.

BIOMACROMOLECULES (2008)

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Article Biophysics

Application and properties of butyl acrylate/pentaerythrite triacrylate copolymers and cellulose-based Granocel as carriers for trypsin immobilization

Jolanta Bryjak et al.

COLLOIDS AND SURFACES B-BIOINTERFACES (2008)

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Article Biochemical Research Methods

In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy

Laurent Geiser et al.

JOURNAL OF CHROMATOGRAPHY A (2008)

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Article Chemistry, Physical

Active Biocatalysts Based on Pepsin Immobilized in Mesoporous SBA-15

Haresh G. Manyar et al.

JOURNAL OF PHYSICAL CHEMISTRY C (2008)

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Article Biochemistry & Molecular Biology

Continuous preparation of two opioid peptides and recycling of organic solvent using liquid/liquid extraction coupled with aluminium oxide column during haemoglobin hydrolysis by immobilized pepsin

Renato Froidevaux et al.

PROCESS BIOCHEMISTRY (2008)

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Review Chemistry, Analytical

Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis

Junfeng Ma et al.

JOURNAL OF SEPARATION SCIENCE (2007)

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Article Biochemical Research Methods

Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries

Jana Krenkova et al.

JOURNAL OF CHROMATOGRAPHY A (2007)

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Review Biotechnology & Applied Microbiology

Improvement of enzyme activity, stability and selectivity via immobilization techniques

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2007)

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Article Multidisciplinary Sciences

Expanding the diversity of chemical protein modification allows post-translational mimicry

Sander I. van Kasteren et al.

NATURE (2007)

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Article Biochemistry & Molecular Biology

Mixed ion exchange supports as useful ion exchangers for protein purification:: Purification of penicillin G acylase from Escherichia coli

Manuel Fuentes et al.

BIOMACROMOLECULES (2007)

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Article Chemistry, Applied

Immobilization of pepsin on chitosan beads

Gamze Durgun Altun et al.

FOOD CHEMISTRY (2007)

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Investigations of reaction kinetics for immobilized enzymess - Identification of parameters in the presence of diffusion limitation

Wouter R. Berendsen et al.

BIOTECHNOLOGY PROGRESS (2006)

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Article Biotechnology & Applied Microbiology

Study of a continuous reactor for selective solvent extraction of haemorphins in the course of peptic haemoglobin hydrolysis

Renato Froidevaux et al.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2006)

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Article Biotechnology & Applied Microbiology

Glyoxyl agarose:: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

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Article Chemistry, Inorganic & Nuclear

Enhancing protease enantioselectivity by ionic liquids based on chiral- or ω-amino acids

Hua Zhao et al.

TETRAHEDRON-ASYMMETRY (2006)

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Article Chemistry, Analytical

Monolithic materials - Promises, challenges, achievements

F Svec et al.

ANALYTICAL CHEMISTRY (2006)

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Article Biochemistry & Molecular Biology

Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces

MT Neves-Petersen et al.

PROTEIN SCIENCE (2006)

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Review Biochemistry & Molecular Biology

Recent trends in protease-catalyzed peptide synthesis

C Lombard et al.

PROTEIN AND PEPTIDE LETTERS (2005)

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Microbial proteases in peptide synthesis: approaches and applications

D Kumar et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2005)

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Article Biotechnology & Applied Microbiology

Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports

F López-Gallego et al.

JOURNAL OF BIOTECHNOLOGY (2005)

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Increasing the binding strength of proteins to PEI coated supports by immobilizing at high ionic strength

BCC Pessela et al.

ENZYME AND MICROBIAL TECHNOLOGY (2005)

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Article Biochemistry & Molecular Biology

Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates

EL Ticu et al.

PROCESS BIOCHEMISTRY (2005)

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Article Polymer Science

Immobilization of pepsin on an acrylamide/2-hydroxyethyl methacrylate copolymer and its use in casein hydrolysis

SP Shukla et al.

JOURNAL OF APPLIED POLYMER SCIENCE (2005)

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Metagenomics and industrial applications

P Lorenz et al.

NATURE REVIEWS MICROBIOLOGY (2005)

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Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports

F López-Gallego et al.

JOURNAL OF BIOTECHNOLOGY (2005)

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Article Biochemistry & Molecular Biology

Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles

L Betancor et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2005)

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Article Chemistry, Multidisciplinary

Free-standing nanogold membranes as scaffolds for enzyme immobilization

S Phadtare et al.

LANGMUIR (2004)

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Article Biochemical Research Methods

Ion exchange using poorly activated supports, an easy way for purification of large proteins

BCC Pessela et al.

JOURNAL OF CHROMATOGRAPHY A (2004)

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Article Biochemistry & Molecular Biology

A kinetic study of bovine haernoglobin hydrolysis by pepsin immobilized on a functionalized alumina to prepare hydrolysates containing bioactive peptides

EL Ticu et al.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2004)

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Article Chemistry, Medicinal

Glide: A new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy

RA Friesner et al.

JOURNAL OF MEDICINAL CHEMISTRY (2004)

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Article Biochemical Research Methods

Affinity chromatography of porcine pepsin and pepsinogen using immobilized ligands derived from the specific substrate for this enzyme

J Frydlová et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2004)

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Article Pharmacology & Pharmacy

Enzyme immobilization onto micro-sized modified PMMA microspheres

SJ Li et al.

METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY (2004)

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Article Biotechnology & Applied Microbiology

Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose.: Full hydrolysis of lactose in milk

C Mateo et al.

BIOTECHNOLOGY PROGRESS (2004)

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Article Biotechnology & Applied Microbiology

Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran

M Fuentes et al.

BIOTECHNOLOGY PROGRESS (2004)

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Article Biology

Use of chemically modified PMMA microspheres for enzyme immobilization

SJ Li et al.

BIOSYSTEMS (2004)

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Review Biochemical Research Methods

Immobilized microfluidic enzymatic reactors

J Krenková et al.

ELECTROPHORESIS (2004)

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Article Biotechnology & Applied Microbiology

Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde

L Betancor et al.

JOURNAL OF BIOTECHNOLOGY (2004)

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Article Food Science & Technology

Continuous production of wheat gluten peptide with foaming properties using immobilized enzymes

H Motoi et al.

EUROPEAN FOOD RESEARCH AND TECHNOLOGY (2004)

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Article Biochemistry & Molecular Biology

N-terminal modifications increase the neutral-pH stability of pepsin

BC Bryksa et al.

BIOCHEMISTRY (2003)

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Article Biochemistry & Molecular Biology

Modified proteases for peptide synthesis in organic media

IY Filippova et al.

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY (2003)

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Article Biotechnology & Applied Microbiology

The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition -: Complete hydrolysis of lactose in dairy products

BCC Pessela et al.

ENZYME AND MICROBIAL TECHNOLOGY (2003)

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Review Biochemical Research Methods

Biotechnological prospects from metagenomics

PD Schloss et al.

CURRENT OPINION IN BIOTECHNOLOGY (2003)

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Article Chemistry, Physical

Direct assembly of gold nanoparticle shells on polyurethane microsphere cores and their application as enzyme immobilization templates

S Phadtare et al.

CHEMISTRY OF MATERIALS (2003)

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Article Chemistry, Multidisciplinary

Gold nanoparticles assembled on amine-functionalized Na-Y zeolite: A biocompatible surface for enzyme immobilization

K Mukhopadhyay et al.

LANGMUIR (2003)

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Article Multidisciplinary Sciences

Dispelling the myths - Biocatalysis in industrial synthesis

HE Schoemaker et al.

SCIENCE (2003)

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Article Chemistry, Multidisciplinary

Protease-catalyzed peptide synthesis on solid support

RV Ulijn et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2002)

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Review Biology

A history of pepsin and related enzymes

JS Fruton

QUARTERLY REVIEW OF BIOLOGY (2002)

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Article Biotechnology & Applied Microbiology

Epoxy sepabeads:: A novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment

C Mateo et al.

BIOTECHNOLOGY PROGRESS (2002)

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Article Gastroenterology & Hepatology

Physiological study of pH stability and sensitivity to pepsin of human gastric lipase

E Ville et al.

DIGESTION (2002)

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Article Biochemistry & Molecular Biology

N-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH

T Tanaka et al.

PROTEIN ENGINEERING (2001)

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Article Biochemistry & Molecular Biology

In vitro refolding of porcine pepsin immobilized on agarose beads

E Kurimoto et al.

JOURNAL OF BIOCHEMISTRY (2001)

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Article Biochemistry & Molecular Biology

Multifunctional epoxy supports:: A new tool to improve the covalent immobilization of proteins.: The promotion of physical adsorptions of proteins on the supports before their covalent linkage

C Mateo et al.

BIOMACROMOLECULES (2000)

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Article Biotechnology & Applied Microbiology

Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit™ L-100

M Sardar et al.

ENZYME AND MICROBIAL TECHNOLOGY (2000)

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Article Multidisciplinary Sciences

Mass spectrometry and immobilized enzymes for the screening of inhibitor libraries

MT Cancilla et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)

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Article Biochemistry & Molecular Biology

Characterization of Alzheimer's β-secretase protein BACE -: A pepsin family member with unusual properties

M Haniu et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2000)

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Article Biotechnology & Applied Microbiology

Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment

C Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2000)

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Article Biotechnology & Applied Microbiology

Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability

R Fernandez-Lafuente et al.

ENZYME AND MICROBIAL TECHNOLOGY (2000)

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Article Chemistry, Applied

Covalent immobilization of α-amylase onto poly(2-hydroxyethyl methacrylate) and poly(styrene-2-hydroxyethyl methacrylate) microspheres and the effect of Ca2+ ions on the enzyme activity

H Tümtürk et al.

FOOD CHEMISTRY (2000)

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Article Chemistry, Multidisciplinary

Encapsulation and biocatalytic activity of the enzyme pepsin in fatty lipid films by selective electrostatic interactions

A Gole et al.

CHEMICAL COMMUNICATIONS (2000)

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