4.7 Article

Combined action of chemical chaperones on stability, aggregation and oligomeric state of muscle glycogen phosphorylase b

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 203, Issue -, Pages 406-416

Publisher

ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.01.106

Keywords

Glycogen phosphorylase b; Mixture of chemical chaperones; Aggregation; Denaturation

Funding

  1. Russian Science Foundation [21-14-00178]
  2. Ministry of Science and Higher Education of Russian Federation [0088-2021-0009]
  3. Russian Science Foundation [21-14-00178] Funding Source: Russian Science Foundation

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Chemical chaperones are small molecules that enhance protein stability and folding. They have significant effects on the stability, oligomeric state, and aggregation of muscle glycogen phosphorylase b (Phb).
Chemical chaperones are a class of small molecules, which enhance protein stability, folding, inhibit protein aggregation, and are used for long-term storage of therapeutic proteins. The combined action of chemical chaperones trehalose, betaine and lysine on stability, aggregation and oligomeric state of muscle glycogen phosphorylase b (Phb) has been studied. Dynamic light scattering data indicate that the affinity of trehalose to Phb increased in the presence of betaine or lysine at both stages (stage of nucleation and aggregate growth) of enzyme aggregation at 48 ?, in contrast, the affinity of betaine to the enzyme in the presence of lysine remained practically unchanged. According to differential scanning calorimetry and analytical ultracentrifugation data, the mixture of trehalose and betaine stabilized Phb stronger than either of them in total. Moreover, the destabilizing effect of lysine on the enzyme was almost completely compensated by trehalose and only partially by betaine. The main protective effect of the mixtures of osmolytes and lysine is associated with their influence on the dissociation/denaturation stage, which is the rate-limiting one of Phb aggregation. Thus, a pair of chaperones affects the stability, oligomeric state, and aggregation of Phb differently than individual chaperones.

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