Drug delivery of sofosbuvir drug capsulated with the β-cyclodextrin basket loaded on chitosan nanoparticle surface for anti-hepatitis C virus (HCV)

The present work-study the improvement of the loading and release efficiency of sofosbuvir drug (SOF) for anti-hepatitis C virus (HCV) by the combination process with beta-cyclodextrin (beta CD) basket to form a novel self-assembly beta CD-SOF which load on the chitosan nanoparticle (Cs NPs) to form a novel hybrid composite (Cs@beta CD-SOF). The characterization process performs for confirming the formation of hybrid composite with various methods. The loading efficiency of SOF is performed by UV-Vis spectroscopy, which is reported at 94.54% for Cs@beta CD-SOF, while in the reverse case the efficiency is beta CD-SOF@Cs 65.2%. The binding constant (Kb) was reported at 1.33 +/- 0.02, and 0.1069 +/- 0.03 min(-1) for Cs@beta CD-SOF and beta CD-SOF@Cs, respectively. The release process of SOF is reported by UV-Vis spectra at 271 nm with 30 min intervals, at pH 7.4 the release efficiency is 67% after 6 h, and 78% after 21 h, while it gave 61% release efficiency at pH 6.8 after time 6 h, and 63% after 21 h. The cytotoxicity assay of the SOF capsulated hybrid materials (beta CD-SOF and Cs@beta CD-SOF) has been detected with three different types of cell lines like mouse normal liver cells (BNL), hepatocellular carcinoma (HepG2), and breast adenocarcinoma (MCF-7). SRB method for the quick screening is used for the cyto-toxicity assay of the SOF capsulated materials, where the examined composites appear a safety status and high viability against the examined cell line. The FRAP method is used to detect the antioxidant activities of SOF capsulated materials. The recommendation for using a safe alternative SOF drug based on Cs NPs and beta CD which give on loading and release efficiency compared to SOF drugs, but the clinical trials are an important step.

Automated summary

This study focuses on improving the loading and release efficiency of sofosbuvir drug (SOF) for anti-hepatitis C virus (HCV) by combining it with beta-cyclodextrin (beta CD) basket to form a novel self-assembly beta CD-SOF. The drug is then loaded onto chitosan nanoparticles (Cs NPs) to create a hybrid composite named Cs@beta CD-SOF. Through various characterization methods, it is confirmed that Cs@beta CD-SOF has a loading efficiency of 94.54% and exhibits high release efficiency.