4.7 Article

Linking the thermostability of FIP-nha (Nectria haematococca) to its structural properties

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 213, Issue -, Pages 555-564

Publisher

ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.05.136

Keywords

Immunomodulatory proteins; Cytotoxicity; Thermostability; Oligomerization

Funding

  1. foundation of the National Key Research and Development Plan [2017YFD0400204]
  2. Dutch Ministry of Agriculture Nature and Food Quality via the knowledge and innovation [KB-37-001-007]
  3. Shanghai Synchrotron Radiation Facility (SSRF)
  4. Tsinghua Uni-versity Branch of China National Center for Protein Sciences

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This study evaluated the correlation between thermal stability and protein structural features of rFIP-nha, which has significant anti-tumor activity. The results showed that higher thermostability was correlated to higher oligomerization states, larger interface area, and more interactions. The findings suggest that the thermostability of FIPs is dependent on their oligomerization state, and is correlated with their cytotoxicity.
Fungal immunomodulatory proteins (FIPs) have been investigated for their use as potential natural derived antitumor molecules. However, the stability of FIPs is critical for their preparation and storage. In this study, the correlation between thermal stability and protein structural features of rFIP-nha, with significant anti-tumor activity, has been evaluated. For comprehensive analysis, FIP-nha and its homologues FIP-gmi, FIP-fve, and LZ-8 were all recombinantly expressed in E. coli. In solution, rFIP-nha and rFIP-gmi formed tetramers; rFIP-fve and rLZ-8 appeared as dimers. Their melting temperatures were 85.1 degrees C, 77.8 degrees C, 66.5 degrees C, and 64.4 degrees C, respectively. Accordingly, their cytotoxicity was also temperature dependent. To investigate the underlying mechanism of their thermostability, we solved the crystal structure of FIP-nha. Detailed structure analysis, molecular dynamic simulation and mutagenesis studies indicated that a higher thermostability was correlated to higher oligomerization states, larger interface area, and more interactions. The structure property studies indicate that Y12, D61 and Y108 were critical for oligomerization and high thermostability of rFIP-nha, but the dimeric and tetrameric states of rFIP-nha exert similar cytotoxicity on A549 cells. Taken together, these findings reveal that thermostability of FIPs was dependent on their oligomerization state, and correlated with their cytotoxicity.

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