Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 208, Issue -, Pages 136-148Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.03.056
Keywords
Mg-doped MBG; OOB protein; Nanofibrous scaffold; ERK1; 2 pathway; Bone tissue engineering
Funding
- National Natural Science Foundation of China [32000961]
- Natural Science Foundation of Hunan Province [2018JJ3598]
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This study discovered a new biomaterial, OOB@MBG-Ca/Mg-3 scaffold, which has multifunctional ability in bone defect repairment by promoting osteoblast differentiation and mineralization through the ERK1/2 pathway and enhancing bone formation in vivo.
Mesoporous bioactive glass (MBG) is a potential biomedical material in bone defect repairment because of its bioactivity, biocompatibility, and osteoinduction properties. Here we report that Mg-doped MBG scaffold with 3:1 Ca/Mg ratio (MBG-Ca/Mg-3) is good for MC3T3-E1 osteoblast differentiation and mineralization. Mimicking bone extracellular matrix structure by electrospinning, we used MBG-Ca/Mg-3 adsorbed with OsteocalcinOsteopontin-Biglycan (OOB), a new unique matrix fusion protein, to form OOB@MBG-Ca/Mg-3 scaffold, which has multifunctional ability in calvarial bone defect repairment in vivo. Intriguingly, we found that OOB@MBG-Ca/Mg-3 scaffold increases the expression of osteoblastic marker genes, including bone morphogenetic protein (Bmp2), osteopontin (Opn), Osterix, Runx2 through activation of ERK1/2. We concluded that OOB@MBG-Ca/Mg-3 scaffold promotes osteoblast differentiation and mineralization through ERK1/2 pathway and it can also enhance bone formation in vivo, which provides a new biomaterial in bone tissue engineering.
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