Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 107, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2022.108654
Keywords
M13 phage; Macrophage; Immunomodulation; Anti-inflammatory
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Funding
- Tarbiat Modares University
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This study found that M13 phages can polarize macrophages towards the M2 phenotype by inducing anti-inflammatory cytokine production and reducing inflammatory cytokine levels. This suggests the potential of M13 phages in immunotherapy.
Bacteriophages are one of the viral components of the human microbiome. M13 phages have recently been considered for immunotherapy because they can be detected by immune cells and stimulated immune responses. Macrophages are essential innate immune cells that respond to stimuli and direct subsequent immune responses. Therefore, it is crucial to evaluate the immunomodulatory effect of phage on macrophage function. For this purpose, peritoneal macrophages from BALB/c and C57BL/6 mice were cultured on the M13 phage, M13 phage-RGD, gelatin-coated, and un-coated wells. Then macrophages were examined for morphological characteristics, L. arginine metabolism, redox potential, inflammatory cytokine production, and phagocytic activity after two and seven days of culture. We observed that M13 phage-coated surfaces induced anti-inflammatory cytokines production and reduced inflammatory cytokines level of BALB/c and C57BL/6 macrophages at the steady-state and post LPS stimulation. In addition, L. arginine metabolism and phagocytic activity of macrophages were directed to the M2 phenotype by induction of arginase-1 and efferocytosis in the M13 phage-containing groups, respectively. The present study confirms the M13 phage's ability to polarize macrophages toward the M2 phenotype. However, using M13 phage in treating inflammatory diseases in animal models could determine their immunotherapy capacity in the future.
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