Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 178, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.indcrop.2022.114632
Keywords
Cellulose; Cellulase; SC-CO2; Enzymolysis; Dextran; Modification enzyme
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Funding
- National Science Foundation of China [31670594, 21908124]
- Qingdao Peoples Livelihood Plan [21-1-4-sf-16-nsh]
- Taishan Scholar Program of Shandong [201511033]
- Talent Fund of Shandong Collaborative Innovation Center of Eco-Chemical Engineering [XTCX 21978041]
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The in-situ homogeneous enzymolysis of cellulose in a supercritical carbon dioxide system using cellulase modified with dextran has been shown to effectively hydrolyze cellulose and produce high concentrations of reducing sugar. This method holds great significance for sustainable utilization of cellulose.
High concentration of reducing sugar from enzymatic hydrolysis of cellulose is the key for utilization of cellulose nowadays. Here, in-situ homogeneous enzymolysis of cellulose in supercritical carbon dioxide (SC-CO2) system by using cellulase modified with dextran (Cell-DEX) could resolve the above question. Cell-DEX can effectively hydrolyze cellulose in SC-CO2 system, and the yield of reducing sugar reaches 242 mg/g cellulose. At the same time, the effects of the modified site, molecular weight of modifier and degree of substitution, stability of Cell-DEX were discussed. Then the enzymolysis of corn straw was also studied in this system and the efficiency has been greatly improved. Therefore, it is an effective way to use cellulose sustainably.
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