Journal
GENOME RESEARCH
Volume 32, Issue 4, Pages 682-698Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.275809.121
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Funding
- Wellcome Trust [202012/Z/16/Z]
- University of Essex
- Queen Mary University of London
- Wellcome Trust [202012/Z/16/Z] Funding Source: Wellcome Trust
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This study uncovers a novel functional role for architectural proteins at TAD borders in Drosophila and establishes a link between TAD reorganization and subsequent changes in gene expression.
The DNA in many organisms, including humans, is shown to be organized in topologically associating domains (TADs). In Drosophila, several architectural proteins are enriched at TAD borders, but it is still unclear whether these proteins play a functional role in the formation and maintenance of TADs. Here, we show that depletion of BEAF-32, Cp190, Chro, and Dref leads to changes in TAD organization and chromatin loops. Their depletion predominantly affects TAD borders located in regions moderately enriched in repressive modifications and depleted in active ones, whereas TAD borders located in euchromatin are resilient to these knockdowns. Furthermore, transcriptomic data has revealed hundreds of genes displaying differential expression in these knockdowns and showed that the majority of differentially expressed genes are located within reorganized TADs. Our work identifies a novel and functional role for architectural proteins at TAD borders in Drosophila and a link between TAD reorganization and subsequent changes in gene expression.
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