Journal
FUNCTIONAL & INTEGRATIVE GENOMICS
Volume 22, Issue 5, Pages 813-824Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-022-00848-6
Keywords
Preeclampsia; Human umbilical cord mesenchymal stem cells; Exosomes; MicroRNA-140-5p; Follistatin-like 3; Angiogenesis; Inflammation
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Funding
- National Key Research and Development Program of China [2018YFC1002804]
- National Natural Science Foundation of China [81771662, 81771618, 81971356]
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This study unraveled the mechanism of action of miR-140-5p transferred by exosomes released from human umbilical cord mesenchymal stem cells (huc-MSCs-EXO) in preeclampsia (PE). It was found that miR-140-5p suppressed PE by repressing FSTL3. Furthermore, huc-MSCs-EXO promoted biological functions and angiogenesis while inhibiting inflammation in hypoxic trophoblast cells.
This work unraveled the action of human umbilical cord mesenchymal stem cells-released exosomes (huc-MSCs-EXO) transfer of miR-140-5p in preeclampsia (PE). miR-140-5p and follistatin-like 3 (FSTL3) expression in placental tissues of PE patients was tested. EXO were isolated from huc-MSCs. Hypoxic trophoblast cells were co-cultured with huc-MSCs-EXO. Cell biological functions, angiogenesis, and inflammation were evaluated. Suppressed miR-140-5p and induced FSTL3 levels were measured in PE. Huc-MSCs-EXO drove biological functions and angiogenesis while hindering inflammation in hypoxic trophoblast cells. Increasing miR-140-5p further improved the positive role of huc-MSCs-EXO for hypoxic trophoblast cells, but the miR-140-5p-mediated effect in hypoxic trophoblast cells was abrogated by overexpressing FSTL3. miR-140-5p from huc-MSCs-EXO suppresses PE through repressing FSTL3.
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