4.5 Article

Characteristics and comparative analysis of Mesona chinensis Benth chloroplast genome reveals DNA barcode regions for species identification

Journal

FUNCTIONAL & INTEGRATIVE GENOMICS
Volume 22, Issue 4, Pages 467-479

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s10142-022-00846-8

Keywords

Mesona chinensis Benth; Chloroplast genome; Comparative analysis; Phylogenetic analysis; Genetic diversity

Funding

  1. Guangxi Innovation-Driven Development Project [GuiKe AA18242040]
  2. China Agriculture Research System [CARS-21]
  3. Scientific Research Funding Project of Guangxi Botanical Garden of Medicinal Plants [GuiYaoJi202011]
  4. Natural Science Foundation of Guangxi [2018JJB130096, 2018GXNSFBA294016]
  5. Guangxi Bagui Scholars and Research Innovation Team Project [GuiYaoChuang2019005]

Ask authors/readers for more resources

Mesona chinensis Benth (MCB) is an important medicinal and edible plant in Southern China and Southeast Asian countries. In this study, the chloroplast (cp) genome of MCB was sequenced and characterized. The results revealed the typical quadripartite structure of the cp genome and identified several highly divergent regions that could be used as DNA barcode regions. Genetic diversity analysis showed a relatively narrow genetic basis of MCB germplasm resources, indicating the urgency and necessity of innovative breeding for MCB.
Mesona chinensis Benth (MCB) is an important medicinal and edible plant in Southern China and Southeast Asian countries. Chloroplast (cp) genome is usually used for plant phylogeny, species identification, and chloroplast genetic engineering. To characterize the cp genome and determine the evolutionary position and perform the genetic diversity analysis of MCB, we sequence and characterize the MCB cp genome. The results show that the cp genome of MCB is a single circular molecule with a length of 152,635 bp. It is a typical quadripartite structure, comprising a large single-copy region (LSC, 83,514 bp) and a small single-copy region (SSC, 17,751 bp) separated by two inverted repeat regions (IRs, 51,370 bp). It encodes 129 unique genes, including 84 protein-coding genes (PCGs), 37 transfer RNAs (tRNAs), and 8 ribosomal RNAs (rRNAs). Altogether 127 simple sequence repeats (SSRs) are identified in the MCB cp genome with 86.61% of mononucleotide repeats. Phylogenetic analysis reveals that MCB is most closely related to Ocimum basilicum based on the whole cp genomes. Several highly divergent regions are found, such as trnH_psbA, rps16_trnQ, trnS_trnG, trnE_trnT, psaA_ycf3, rp132_trnL, ccsA_ndhD, ndhG_ndhI, and rps15_ycf1, which can be proposed for use as DNA barcode regions. Genetic diversity analysis unveils a relatively narrow genetic basis of MCB germplasm resources. Therefore, the innovative breeding of MCB is very urgent and necessary in future research.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available