Journal
FUEL
Volume 314, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.fuel.2021.122738
Keywords
Biofuel; CRISPR; Diatom; Gene; Lipid; Nutrient stress
Categories
Funding
- CEFIPRA Indo French project for Pre doctoral fellowships
- Indo-French Centre for the Promotion of Advanced Research (IFCPAR/CEFIPRA) Indo France [PPMB-7133/2020]
- Department of Science and Technology Nanomission Government of India [SR/NM/NT-1090/2014]
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Microalgae, especially diatoms, are abundant sources of oil for biofuel production. This review focuses on enhancing lipid accumulation in diatoms through biochemical modeling and genetic engineering, as well as exploring the use of molecular tools, like CRISPR, for targeted genome modification to increase oil production. The aim is to widen the scope of metabolic pathways, improve lipid and biofuel production under nutrient stress, and study the quality and life cycle assessment of lipids from diatoms.
Microalgae are microscopic plants which are found in water, snow as well as land. They are one of the major resources of biofuel. However, the phenomenon of biolipid accumulation and its downstream processing into biofuel for commercialization and industrialization has yet to be standardized at economical scale. Among microalgae, diatoms are third generation microalgae which produces abundant oil and thus serves as one of the biggest sources of fossil fuel energy. They account for more than 25% of global biomass production. Diatoms would suffice world's energy crisis if they are milked/harvested for oil without being sacrificed. Simultaneously in order to get benefitted for crude oil the biochemical modeling of oleaginous microalgae would help in increasing its lipid accumulation to be able to be used in diatom solar panels for DiafuelTM (biofuel from diatoms) production. Such types of living algal solar panels grow in the presence of nitrogen, phosphorus, potassium, silicates, trace metals, few other micro nutrients and even in wastewater. Additionally, molecular tools like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) offers targeted genome modification in diatoms for increasing oil production deploying specific genes. The review adds the scope to unravel such techniques in diatoms to harvest lipid for DiafuelTM production from a wide range of diatom strains. Several such genetically modified or naturally selected diatom strains rich in oil serve as an important ingredient for diatom solar panels. The main target of this review is to widen the scope of metabolic pathways for enhancing lipid and biofuel in diatoms under nutrient stress media and adapting genetic engineering tools to identify genes responsible for them. It also targets to study the quality of biofuel and life cycle assessment of lipids from diatoms.
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