Transcriptome and metabolite integrated analysis reveals that exogenous ethylene controls berry ripening processes in grapevine

Although grapevine (Vitis vinifera L.) is generally classified as a non-climacteric fruit, the regulatory mechanisms of ethylene in the ripening of non-climacteric fruit are still poorly understood. In this study, exogenous ethephon (ETH) strongly stimulated fruit color and anthocyanin accumulation, which was consistent with the increased expression of anthocyanin structural, regulatory, and transport genes. ETH application increased ABA content and decreased IAA content by coordinating ABA and auxin biosynthesis regulatory network. ETH treatment also accelerated sugar (glucose and fructose) accumulation by enhancing the gene expression involved in sugar transport and sucrose cleavage. ETH treatment blocked the synthesis of cellulose and accelerated the degradation of pectin, which was strongly associated with berry softening. To further confirm the function of ethylene biosynthesis and signaling genes, transient overexpression of VvACO4 and VvEIL3 were performed in both in tomato and strawberry fruits. These findings of the ethylene cascade add to our understanding of ethylene in non climacteric berry ripening regulation and revealed a complex involvement of ethylene and its interplay with phytohormones during grapevine berry ripening.

Automated summary

This study reveals the regulatory mechanisms of ethylene in the ripening of non-climacteric grapevine berries. Ethylene treatment stimulates fruit color, anthocyanin accumulation, ABA biosynthesis, and sugar accumulation, while inhibiting cellulose synthesis and promoting pectin degradation. The study also confirms the involvement of ethylene biosynthesis and signaling genes in fruit ripening.