Journal
FOOD CONTROL
Volume 136, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2022.108824
Keywords
Digital microfluidic; Loop-mediated isothermal amplification; Foodborne pathogen; Multiplex detection
Categories
Funding
- Key Laboratory of Agricultural Product Quality and Food Safety of Zhuhai [R1053]
- Zhuhai industryuniversity-research cooperation project [ZH22017002200012PWC]
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A method using a digital microfluidic chip and loop-mediated isothermal amplification technology was developed for multiplex detection of foodborne pathogens. The method showed higher sensitivity, simpler operation, higher efficiency, and smaller size compared to the commercial benchtop LAMP method.
Rapid detection of foodborne pathogens is critical to assure food quality. In this study, a digital microfluidic (DMF) chip in combination with loop-mediated isothermal amplification (LAMP) was developed for multiplex detection of four common foodborne pathogens. The DMF chip contains ten reaction chambers with dehydrated primers targeting different genes for simultaneous amplification by LAMP in the volume of 2 mu l/reaction with a detection limit of 10(2) CFU/ml for all target bacteria according to the 3s criteria. Using spiked milk for validation, as low as 10(3) CFU/ml of all four pathogenic bacteria were detected simultaneously in a single test within 50 min. The proposed method exhibited higher sensitivity to the commercial benchtop LAMP method with the advantages of simpler operation, high efficiency, and miniaturization, showing great potential for point-of-care (POC) testing of various foodborne pathogens.
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