Journal
FOOD CHEMISTRY
Volume 371, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131097
Keywords
Pectic oligosaccharides; High hydrostatic pressure; Tri-galacturonic acid; Allergy
Funding
- National Natural Science Fund [31601395]
- National Key Research and Development Program of China [2018YFC1602201]
- Key Program for Shaanxi Science and Technology [2020NY-146]
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The study explored the preparation of pectic oligosaccharides (POS) using high hydrostatic pressure-assisted enzymatic (E-HHP) method. It was found that TriGalA and POS could attenuate the release of beta-hexosaminidase and histamine, reduce the production of IL-4, and inhibit extracellular Ca2+ influx of RBL-2H3 cells, indicating POS as a potential inhibitor in regulating mast cell-mediated allergic inflammatory responses.
The conditions for the preparation of pectic oligosaccharides (POS) by high hydrostatic pressure-assisted enzymatic (E-HHP) method were explored. The optimal conditions consisted of the pressure of 350 MPa for 20 min, and enzymolysis for 60 min with 0.011 U/mL enzyme. The products were isolated by ion exchange chromatography, galacturonic acid, di- and tri-galacturonides (Tri-GalA) with high purity were obtained. Additionally, the effects of POS on activation and degranulation of RBL-2H3 mast cells were investigated. It was found that TriGalA and POS could attenuate the release of beta-hexosaminidase and histamine, reduce the production of IL-4 and inhibit the extracellular Ca2+ influx of RBL-2H3 cells. Notably, 150 mu g/mL POS significantly alleviated the IgEmediated allergic reaction of RBL-2H3 cells. These results indicate that POS could be used as an inhibitor in regulating mast cell-mediated allergic inflammatory responses.
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