4.7 Article

Quantum dot microsphere-based immunochromatography test strip enabled sensitive and quantitative on-site detections for multiple mycotoxins in grains

Journal

FOOD CHEMISTRY
Volume 376, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131868

Keywords

Immunochromatography test strip; Quantum dot microsphere; Multiple mycotoxin detection; On-site detection

Funding

  1. National Natural Science Foundation of China [32000028]
  2. One belt, one road, innovation and cooperation project, a policy guidance program (International Sci-ence and technology cooperation) by Jiangsu Province [BZ2019013]
  3. China Postdoctoral Science Foundation [2020M681649]
  4. Jiangsu Agricultural Science and Technology Innovation Fund [SCX (19) 3014]
  5. Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD]

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This work proposes a sensitive and quantitative on-site detecting solution for mycotoxins, which can be quickly completed on site through immunochromatography test strips, with high accuracy and good selectivity.
In this work, we design a sensitive and quantitative on-site detecting solution for Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) as often found in moldy grains and harmful to human health. Using quantum dot microsphere-based immunochromatography test strip, the proposed method can sensitively detect AFB1, OTA and ZEN in low detection limits of 0.01 ng/mL, 0.2 ng/mL and 0.032 ng/mL, and quantitatively measure their concentrations from 0.01 ng/mL to 1 ng/mL, from 0.2 ng/mL to 200 ng/mL and from 0.032 ng/mL to 32 ng/mL in high accuracy and good selectivity. More importantly, these multiple mycotoxin detections only relying on simple manual operations and portable handheld test strip reader can be finished on site within 45 min. Therefore, the proposed method is a promising solution supporting sensitive and quantitative on-site detections for multiple mycotoxins.

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