Journal
FOOD CHEMISTRY
Volume 380, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132183
Keywords
Myoglobin; In vitro digestion; LC-MS; MS; Digestibility; Molecular dynamics simulation; Mechanisms
Funding
- National Natural Science Foundation Council [32072211, 31530054]
- Jiangsu Province Department of Education (Innovation Group of Meat Nutrition andBiotechnology)
- Ministry of Science and Technology (Ten Thousand Talent)
Ask authors/readers for more resources
This study investigated the structural changes of myoglobin after salt treatment and the binding mode of substrate and pepsin/trypsin. The results showed that salt treatment altered the heme structure and hydrophobic cavity of myoglobin, resulting in reduced protein digestibility. Additionally, trypsin was found to be more effective in digesting high molecular weight peptides. Molecular dynamics simulation revealed that the binding between myoglobin and pepsin/trypsin was mainly maintained by hydrogen bond and hydrophobicity. The findings provide new insights into the low bioavailability of myoglobin.
ABSTR A C T In this paper, the structural changes of myoglobin after salt treatment and the binding mode of substrate and pepsin/trypsin were studied by ultraviolet absorption spectroscopy, synchronous fluorescence spectroscopy, LC-MS/MS and molecular dynamics simulation. NaCl treatment changed the heme structure and hydrophobic cavity of myoglobin, which in turn decreased the digestibility of the protein. Trypsin was more effective for digestion of high molecular weight peptides. Molecular dynamics simulation showed that the binding of myoglobin and pepsin/trypsin was mainly maintained by hydrogen bond and hydrophobicity. In addition, the catalytic active center of pepsin is not suitable for long-time existence of low-molecular weight peptide under salt treatment. The findings provide a new insight into the low bioavailability of myoglobin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available