Rapid and sensitive detection of clomazone in potato and pumpkin samples using a gold nanoparticle-based lateral-flow strip

In this study, an ultrasensitive monoclonal antibody (mAb) was produced and used to develop a gold nanoparticle-based lateral flow immunochromatographic (ICA) strip for screening of clomazone (CLO) in potato and pumpkin samples. With assayed by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method, the mAb belonging of IgG2 subclass showed a half-maximal inhibitory concentration (IC50) of 3.47 ng/ mL and a linear range of detection of 0.43-28.09 ng/mL. A cross-reactivity test revealed that the mAb had good specificity for CLO. The strip assay had a visual limit of detection (LOD) of 5 mu g/kg and a cut-off value of 50 mu g/ kg for CLO pumpkin samples (potato samples was 100 mu g/kg) when evaluated with the naked eye. The results were consistent with ic-ELISA and high performance liquid chromatography tandem mass spectrometry (HPLCMS). Thus, this ICA strip assay represents a potentially tool for on-site and rapid initial detection of CLO in potato and pumpkin samples.

Automated summary

A highly sensitive monoclonal antibody was developed to detect clomazone in potato and pumpkin samples using a gold nanoparticle-based lateral flow immunochromatographic strip. The method showed good specificity and reliability, making it a potentially valuable tool for on-site and rapid initial screening of clomazone.