Journal
FOOD CHEMISTRY
Volume 373, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131490
Keywords
Ambient Mass Spectrometry; Mass Spectrometry Imaging; LAESI; Patulin; Mycotoxins; MCR-ALS
Funding
- Brazilian Federal Agency for Support and Evaluation of Grad-uate Education (CAPES)
- Brazilian Research Council CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
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This study utilized LAESI imaging to investigate the diffusion of the mycotoxin patulin from rotten to healthy areas of fruits. Patulin was detected in all mold-infected fruits, and LAESI images demonstrated the diffusion of patulin from the rotten area to unaffected parts of the fruits. The study highlights the advantage of LAESI imaging over traditional analytical methods for studying mycotoxin diffusion in fruits.
This work describes the use of laser ablation electrospray ionization mass spectrometry imaging (LAESI imaging) to investigate the diffusion of the mycotoxin patulin from rotten to healthy areas of fruits. Slices of mold-infected and uninfected (control) apples and strawberries were prepared, and this was the only sample preparation step used. An infrared laser beam (2.94 mu m) was used to irradiate the slices, resulting in the ablation of sample compounds directly ionized by electrospray and analyzed by mass spectrometry. Multivariate curve resolution - alternating least squares was applied in unfolded LAESI images to obtain relative quantity information. Patulin was not detected in the control samples but was seen in all mold-infected fruits. LAESI images showed the diffusion of patulin from the rotten area to unaffected parts of the fruits. This study points out the advantage of LAESI imaging over traditional analytical methods used to study the diffusion of mycotoxins in fruits.
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