Polydopamine-coated magnetic Spirulina nanocomposite for efficient magnetic dispersive solid-phase extraction of aflatoxins in pistachio

An efficient adsorbent was synthesized and used in magnetic dispersive solid phase extraction (MDSPE) of aflatoxins B1, B2, G1, and G2 at trace levels in pistachio prior to analysis by HPLC equipped with a fluorescence detector. Spirulina (Sp) algae was first magnetized, followed by surface modification with dopamine (Dp). The adsorbent was characterized using FT-IR, XRD, FE-SEM, EDX, VSM, and BET analyses. The effects of different analytical parameters on the extraction performance were evaluated. Under optimal conditions, good limits of detection (LODs) and quantifications (LOQs) were achieved in the ranges of 0.02-0.07 and 0.06-0.21 ng g(-1), respectively. The RSDs were 5.9, 6.3, 5.6, and 7.3% for AFB1, G1, B2, and G2, respectively. The proposed method was successfully used to determine AFs in pistachio samples and acceptable recoveries in the range of 72-95% were obtained.

Automated summary

An efficient adsorbent was synthesized and applied in magnetic dispersive solid phase extraction of aflatoxins in pistachio, achieving good limits of detection and quantification under optimal conditions. The proposed method successfully determined the presence of aflatoxins in pistachio samples with acceptable recovery rates.