Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 33, Issue 1, Pages 346-359Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2022.2076812
Keywords
Okadaic acid; marine biotoxin; monoclonal antibody; immunoassay; ELISA
Funding
- Hainan Provincial Science and Technology Special Fund [ZDYF2022SHFZ082]
- Natural Science Foundation of Hainan Province [821RC556]
- special fund of the State Key Joint Laboratory of Environmental Simulation and Pollution Control from Tsinghua University [20K10ESPCT]
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The study reported a highly sensitive monoclonal antibody against okadaic acid (OA) and developed a more sensitive indirect competitive ELISA (icELISA) for rapid detection of OA.
Okadaic acid (OA), one of marine biotoxins produced by several species of dinoflagellates, can accumulate in marine animals. The consumption of OA-contaminated seafood can cause diarrhoeic shellfish poisoning. Many countries have established regulatory restriction to limit the level of OA in seafood. In the present study, we report a highly sensitive monoclonal antibody (mAb) against OA produced by a new immunogen. Two monoclonal hybridoma cell lines (M8 and M12) that producing mAb with the best sensitivity and specificity were obtained. The obtained mAb-M12 was used to develop an indirect competitive ELISA (icELISA) with 50% inhibition concentrations of 0.15 ng mL(-1) and a working range of 0.02-2.74 ng mL(-1). The developed icELISA was more sensitive than the previous reports. HPLC-MS confirmed the accuracy and the working range of icELISA, suggesting that the developed icELISA is suitable for the rapid detection of OA in oysters and green mussels.
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