4.5 Article

The BRCA2 and CDKN1A-interacting protein (BCCIP) stabilizes stalled replication forks and prevents degradation of nascent DNA

Journal

FEBS LETTERS
Volume 596, Issue 16, Pages 2041-2055

Publisher

WILEY
DOI: 10.1002/1873-3468.14406

Keywords

BCCIP; genome stability; replication fork; replication stress

Funding

  1. India Alliance DBT Wellcome Intermediate fellowship [IA/I/16/1/502365]

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DNA replication stress, characterized by impaired replication fork progression, can lead to genomic instability. The protein BCCIP plays a crucial role in protecting nascent DNA strands from degradation during replication stress.
DNA replication stress is characterized by impaired replication fork progression, causing some of the replication forks to collapse and form DNA breaks. It is a primary cause of genomic instability leading to oncogenic transformation. The repair-independent functions of the proteins RAD51 and BRCA2, which are involved in homologous recombination (HR)-mediated DNA repair, are crucial for protecting nascent DNA strands from nuclease-mediated degradation. The BRCA2 and CDKN1A-interacting protein (BCCIP) associates with BRCA2 and RAD51 during HR-mediated DNA repair. Here, we investigated the role of BCCIP during the replication stress response. We find that in the presence of replication stress, BCCIP deficiency increases replication fork stalling and results in DNA double-strand break formation. We show that BCCIP is recruited to stalled replication forks and prevents MRE11 nuclease-mediated degradation of nascent DNA strands.

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