Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 173, Issue -, Pages 92-102Publisher
ELSEVIER
DOI: 10.1016/j.ejpb.2022.02.019
Keywords
Mucus; Mucin; Multi-omics; Rheology; cryoSEM; Dog
Categories
Funding
- VINNOVA [2019-00048]
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The study aimed to characterize the composition and structure of gastrointestinal mucus in dogs. It was found that mucus mainly consists of water and maintained a pH around 6.5. The protein composition of mucus differed between the stomach and intestines, while lipid and metabolite abundance was higher in the jejunal mucus. Microscopic analysis showed smaller pore size in small intestinal mucus compared to the large intestine. Overall, mucus is a highly viscous and hydrated material.
Gastrointestinal (GI) mucus is continuously secreted and lines the entire length of the GI tract. Essential for health, it keeps the noxious luminal content away from the epithelium. Our aim was to characterize the composition and structure of mucus throughout the various GI segments in dog. Mucus was collected from the stomach, small intestine (duodenum, jejunum, ileum), and large intestine (cecum, proximal and distal colon) from dogs. Composition was determined by multi-omics. Structural properties were investigated using cryoSEM and rheology. GI mucus contained 74-95% water and maintained a pH around 6.5. The proteome was similar across the different GI segments. The highest abundant secreted gel-forming mucin in the gastric mucus was mucin 5AC, whether mucin 2 had highest abundance in the intestinal mucus. Lipid and metabolite abundance was generally higher in the jejunal mucus than the colonic mucus. CryoSEM microscopy revealed smaller pore size in small intestinal mucus, which increased in the large intestine. All mucus samples showed shear-thinning behavior and characteristics of gel-like structure. In conclusion, the mucus is a highly viscous and hydrated material. These data provide an important baseline for future studies on human and canine intestinal diseases and the dog model in drug absorption.
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