Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 174, Issue -, Pages 47-55Publisher
ELSEVIER
DOI: 10.1016/j.ejpb.2022.03.013
Keywords
Hepatocellular carcinoma; Gene silence; Carbon dots; siRNA
Categories
Funding
- Doctoral Scientific Research Foun-dation of Xinxiang Medical University [505143]
- Key Scientific Research Project of Higher Education Institutions of Henan Province [14A180035]
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Researchers synthesized fluorescent carbon dots as a gene vector and resolved the cell targeting issue by using aptamer AS1411. The siRNA delivery system effectively silenced gene expression and inhibited the migration and invasive propensity of hepatocellular carcinoma cells. This study provides a potential efficient method for gene delivery and therapy.
Using small interfering RNA (siRNA) for the specific gene-silencing has been a novel therapeutic method for the treatment of incurable diseases such as malignancies. However, it remains a challenge whether siRNA can be safely and effectively delivered into target cells. Therefore, we synthesized fluorescent carbon dots (CDs) as a gene vector at the siRNA delivery system that induced efficient gene knockdown in vitro while binding aptamer AS1411 to resolve the difficulty in cell targeting. We found that CDs with adequate biocompatibility can improve the efficiency of cellular uptake of siRNA. CLSM and FCM results showed that CDs were mainly localized in the cytoplasm and emitted bright green fluorescence. In addition, the CD/siRNA delivery system mediated by the aptamer AS1411 effectively silenced the expression of Fragile X mental retardation protein (FMRP) and successfully inhibited the migration and invasive propensity of hepatocellular carcinoma (HCC) cells. In summary, we have synthesized a valuable siRNA delivery vector enabling not only bioimaging but also effective downregulation of gene expression, which is indicative of an efficient potential for gene delivery and therapy.
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