Responses of submerged macrophyte Ceratophyllum demersum to the gradient concentrations of microcystin-LR (MC-LR)

The responses of Ceratophyllum demersum to gradient concentrations (0, 0.8, 3.2, and 10 mu g/L) of microcystin-LR (MC-LR) were comprehensively investigated by laboratory simulation experiments. The high reduction and accumulation efficiency of MC-LR by C. demersum were verified in this study. Results showed that the reduction ratio of MC-LR in the cultivation medium was up to 99% after 14 days of exposure, and the accumulation of MC-LR in C. demersum was highest at an exposure concentration of 10 mu g/L, the value of which was 0.9 ng/g fresh weight (FW). Meanwhile, a series of negative effects on C. demersum was detectable, accompanied by a significant biomass reduction of the plant and changes in microbial community composition. In particular, this study indicated that the amount of Flavobacteria was elevated under the stress of MC-LR, provoking great threats to aquatic ecosystems. Moreover, oxidative damage was evidenced by the changes in total antioxidant capacity, superoxide dismutase, and glutathione. The results also demonstrated significant increases in sugar (0.025 mg/g FW), protein (0.3 mg/g FW), and carotenoids (0.6 mg/g FW) in C. demersum stressed by 10 mu g/L of MC-LR, compared with the control without microcystins, which were among the defense strategies for dealing with adverse conditions. These results verified the good potential of submerged macrophytes as an eco-friendly strategy for controlling cyanobacterial blooms. However, the negative effects of MC-LR on the macrophytes themselves were also demonstrated, which would be considered in future practice and management.

Automated summary

This study comprehensively investigated the responses of Ceratophyllum demersum to gradient concentrations of microcystin-LR (MC-LR), revealing its high reduction and accumulation efficiency of MC-LR. It also highlighted the negative effects on C. demersum, such as biomass reduction and changes in microbial community composition, as well as oxidative damage and altered defense strategies. Furthermore, it verified the potential of submerged macrophytes as an eco-friendly strategy for controlling cyanobacterial blooms, while considering the negative effects on the macrophytes themselves.