Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 29, Issue 42, Pages 63127-63140Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-022-20166-5
Keywords
DNA damage; Binding affinity; Long-chain fatty acids; Chromosomal aberrations; Mitotic index; Molecular docking; Pethoxamid
Categories
Ask authors/readers for more resources
Pethoxamid is a chloroacetamide herbicide commonly used to kill weeds in crops. This study investigated the cytotoxic and genotoxic effects of pethoxamid on Allium cepa cells. The results showed that pethoxamid inhibited the root growth of onion and caused chromosomal aberrations and DNA damage. Molecular docking analysis revealed that pethoxamid binds selectively to GC-rich regions in DNA and has a high affinity for VLCFA synthases. Therefore, the genotoxicity and cytotoxicity of pethoxamid may be attributed to its multiple binding ability with DNA and VLCFA synthases.
Pethoxamid is chloroacetamide herbicide. Pethoxamid is commonly used to kill different weeds in various crops. Pethoxamid can leach in the water and soil and can cause toxic effects to other non-target species. Current study is therefore aimed to perform the investigation of the cytotoxic and genotoxic effects of pethoxamid on Allium cepa cells.The root growth, mitotic index (MI), chromosomal aberrations (CAs), and DNA damage were assessed through root growth inhibition, A. cepa anatelophase, and alkaline comet assays, respectively. Furthermore, molecular docking was performed to evaluate binding affinity of pethoxamid on DNA and very-long-chain fatty acid (VLCFA) synthases. In root growth inhibition test, onion root length was statistically significantly decreased in a concentration dependent manner. Concentration- and time-dependent decreases in MI were observed, whereas increase in CAs such as disturbed ana-telophase, chromosome laggards, stickiness, anaphase bridges, and DNA damage was caused by the pethoxamid on A. cepa root cells. Molecular docking revealed that pethoxamid binds selectively to GC-rich regions in the minor groove of the DNA structure and showed remarkable binding affinity against all synthases taking part in the sequential biosynthesis of VLCFAs. It was concluded that the pethoxamid-induced genotoxicity and cytotoxicity may be through multiple binding ability of this herbicide with DNA and VLCFA synthases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available