Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 196, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2022.104805
Keywords
NAC transcription factor; Drought stress; TaNAC5D-2; BSMV-VIGS
Categories
Funding
- State Key Laboratory of Crop Stress Biology for Arid Areas, NWAFU, Yangling, Shaanxi, China [CSBAA2020005]
- National Key Research and Development Program of China [2017YFD0301101, 2016YFD0300203-3]
- Key Project of Science and Technology of Henan Province [182102110148]
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In this study, a drought-responsive NAC gene, TaNAC5D-2, was isolated and characterized in wheat. The results showed that TaNAC5D-2 functions as a positive regulator of drought tolerance through abscisic acid-mediated stomatal closure.
NAC transcription factors play critical roles in regulating drought stress. However, the drought-responsive NAC transcription factors are largely unexplored in wheat. In this study, we isolated a NAC gene, TaNAC5D-2, and characterized the function of TaNAC5D-2 as a positive regulator of drought tolerance. The expression level of TaNAC5D-2 was induced under drought or abscisic acid treatment. Transient expression and transcriptional activity assays showed that TaNAC5D-2 is localized to the nucleus, and the C-terminal region exhibited transcriptional activity. Overexpression of TaNAC5D-2 in Arabidopsis thaliana led to greater drought tolerance, fresh weight and dry weight than wild-type plants, and the water loss was significant inhibited in the leaves of overexpression lines. Conversely, silencing of TaNAC5D-2 in wheat seedlings led to increased water loss and decreased growth compared to control and negative control plants under drought stress. RNA-Seq and RT-qPCR revealed that many Group A PP2C genes were upregulated in the TaNAC5D-2 silenced plants compared to negative control. And silencing TaNAC5D-2 negatively regulate ABA-induced stomatal closure that led to higher water loss. These data suggest that TaNAC5D-2 acts as a drought-responsive regulator, possibly by an abscisic acid-mediated stomatal closure to control water loss under drought condition.
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