Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 198, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2022.104847
Keywords
Ammonium; Nitrate; Root growth; Biomass; Rice; Nitrogen assimilation; Nitrate transporters
Categories
Funding
- Department of Biotechnology (DBT) India
- CSIR
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Nitrogen uptake and assimilation are crucial for plant growth and productivity. This study evaluated eleven rice genotypes under sufficient and low nitrogen conditions, and identified a genotype associated with enhanced nitrogen levels and assimilation. The findings provide important insights for breeding nitrogen use-efficient rice varieties.
Nitrogen (N) uptake and its assimilation are crucial steps for plant growth and productivity. Plant's N balance largely depends on nitrate (NO3-) and ammonium (NH4+) forms present in the rhizosphere. Due to the fluctuating and heterogeneous availability of these N forms in the soils, plants encounter low to N deficiency. In contrast to low nitrogen, high N in the form of ammonium (NH4+) severely hampers plant development and causes NH4+ toxicity. In this study, we assessed eleven rice genotypes under sufficient (SN) and low N (LN) conditions. From the analysis, we identified a rice genotype, PB1, which is hypersensitive to SN and showed reduced root and shoot growth. In contrast to the SN condition, PB1 showed improved growth performance under the LN con-dition. Our data show that compromised growth of PB1 under SN condition is associated with increased activity of N responsive genes such as OsAMT1.1, OsAMT2.3, OsAMT3.1 and OsAMT3.2, OsNRT1.1A and OsNRT1.1B. Strikingly, LN treatment improved the root and shoot biomass with a concomitant increase in levels of NO3- and NH4+ transporter genes along with an increase in shoot: root NO(3)(- )ratio. Additionally, we show that increased levels of N in PB1 under SN condition are associated with the enhanced activity of the GS-GOGAT pathway. Further, our ionomic analysis highlighted the role of N-defined Fe accumulation which is partially associated with the N toxicity. Taken together, our study led to identifying a rice genotype (Oryza sativa L.) which is associated with enhanced N levels and assimilation and could be used for raising N use efficient rice varieties using breeding approaches.
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