Journal
ELECTROCHIMICA ACTA
Volume 409, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2022.139968
Keywords
Disposable devices; Screen-printed carbon electrodes; Electroanalysis; Uric acid; Tyrosinase-biosensor
Categories
Funding
- CNPq [303338/2019-9]
- CAPES [001, 88887.504861/2020-00]
- FAPESP [2019/23177-0, 2017/21097-3]
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This paper presents a novel water-based conductive ink and successfully applies it in the screen-printing of disposable electrodes. The electrode shows good linear relationship and low detection limit in the quantification of uric acid and catechol. The new electrode demonstrates outstanding analytical characteristics and has the potential to be a disposable, low-cost, and environmentally friendly platform for electrochemical sensing and biosensing purposes.
This paper presents a novel water-based conductive ink obtained by proper combination of chitosan (C) biopolymer, graphite (G) powder, and glycerol (G), and its subsequent use in the screen-printing of disposable electrodes on polyethylene terephthalate (PET) plastic obtained from recyclable soda bottles (CGG/PET electrodes). The electrode was used in two directions, as a sensor for the quantification of uric acid and as a biosensor for the enzymatic quantification of catechol. The linear sweep voltammetrydetection of uric acid (UA), showed a linear range between 8.0 and 500 mu mol L-1 and a limit of detection (LOD) of 0.36 mu mol L-1. Additionally, the performance of the resulting electrodes for biosensing purposes was evaluated through the development of a catechol biosensor by the modification of the GCG/PET surface with the tyrosinase (Tyr) enzyme, multiwalled carbon nanotubes (MWCNTs), and dyhexadecyl phosphate (DHP) (Tyr-MWCNT-CGG/PET). Using the chronoamperometry technique a linear relationship from 0.5 to 50 mu mol L-1 and LOD of 0.3 mu mol L-1 were achivied for catechol. The proposed electrode has demonstrated outstanding analytical characteristics, proving its potential as a disposable, low-cost, and environmentally friendly platform for electrochemical sensing and biosensing purposes. (C) 2022 Elsevier Ltd. All rights reserved.
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