Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds

Article Biophysics

Confined electrochemiluminescence imaging microarray for high-throughput biosensing of single cell-released dopamine

Ningning Wang et al.

Summary: In this work, a CEIM chip was designed for high-throughput quantification of cell-secreted dopamine. The chip was able to recognize the target DA secretion from cells and emit an ECL signal without the need for additional coreactants.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Chemistry, Analytical

Electrochemiluminescence Microscopy of Cells: Essential Role of Surface Regeneration

Dongni Han et al.

Summary: Electrochemiluminescence (ECL) microscopy is successfully used for imaging cells, micro-/nano-objects, and electrochemical processes. The decrease of ECL signal during successive image recordings is a key limitation for ECL microscopy development. An electrochemical approach involving cathodic regenerative treatment can restore the ECL signal intensity, allowing for continuous image recording without signal loss.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Electrochemiluminescence Loss in Photobleaching

Dongni Han et al.

Summary: The study investigated the effects of photobleaching on electrochemiluminescence (ECL) for the first time, demonstrating a linear correlation between ECL decrease and photoluminescence (PL) loss. The presented strategy provides valuable information on ECL excited state fundamentals and opens new opportunities for exploring cellular membranes by combining ECL microscopy with photobleaching techniques such as FRAP or FLIP methods.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

Bio-Coreactant-Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport

Cheng Ma et al.

Summary: The bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy allows for ECL imaging of intracellular structure and dynamic transport by using Ru(bpy)(3)(2+) and intracellular biomolecules. The research reveals the universal edge effect of cellular electroporation, as well as the dynamic transport of Ru(bpy)(3)(3+) in different cellular compartments and its correlation with the actin cytoskeleton.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Biophysics

Dual-signal electrochemiluminescence immunosensor for Neuron-specific enolase detection based on dual-potential emitter Ru(bpy)32+functionalized zinc-based metal-organic frameworks

Xue Dong et al.

Summary: A dual-signal ratiometric electrochemiluminescence sensing strategy based on Ru-MOF-5 nanoflowers was developed for sensitive detection of NSE. By monitoring the dual-signal ECL and calculating their ratios, precise analysis of NSE was achieved, reducing system error and background signal.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Multidisciplinary

Spatially Selective Imaging of Cell-Matrix and Cell-Cell Junctions by Electrochemiluminescence

Hao Ding et al.

Summary: A novel method for spatially selective imaging of cell junctions by regulating the thickness of the electrochemiluminescence (ECL) layer through control of luminophore and co-reactant concentrations has been reported. Low luminophore concentration reveals cell-matrix adhesions, while high concentration allows sequential imaging of cell-matrix and cell-cell junctions. This strategy not only provides new insights into ECL mechanisms but also expands the potential applications of ECL microscopy in bioimaging.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

Single Biomolecule Imaging by Electrochemiluminescence

Yujie Liu et al.

Summary: This study successfully imaged single biomolecules using RuDSNs/AuNPs as ECL nanoemitters, demonstrating the visualization of single protein molecules and single membrane proteins in cells, overcoming the challenges in ultrasensitive ECL analysis and providing valuable information for cellular biology research.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Electrochemistry

Electrocatalyst Screening on a Massive Array of Closed Bipolar Microelectrodes

Todd J. Anderson et al.

Summary: In this study, a novel electroanalytical platform utilizing bipolar ultramicroelectrode arrays and electrochemiluminescence imaging was developed for rapid screening of electrocatalysts. By electrodeposition of a radial gradient of catalytic metal on carbon-gold bipolar UME arrays, the impact of varying Ni(OH)2 coverage on the catalytic activity for the hydrogen evolution reaction (HER) was investigated. Future work will focus on expanding this platform to screen various metal alloys by incorporating multiple metal salts delivery during the gradient electrodeposition process.

JOURNAL OF THE ELECTROCHEMICAL SOCIETY (2021)

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Article Chemistry, Multidisciplinary

Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Yumeng Ma et al.

Summary: This study introduces a label-free shadow electrochemiluminescence (ECL) microscopy technique to image single living mitochondria deposited on electrode surface. By utilizing negative optical contrast and local diffusional hindrance, this approach visualizes individual mitochondria and validates their functionality with classical fluorescent biomarkers. The versatility and extreme sensitivity of this method make it promising for imaging subcellular structures while alleviating photobleaching and phototoxicity issues associated with conventional microscopy.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Biophysics

Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps

Kaoru Hiramoto et al.

Summary: This study demonstrates the electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time, providing visualization of O2 distribution and enabling time-lapse imaging for studying cellular dynamics in spheroids with promising high-throughput imaging strategy.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Electrochemistry

Electrogenerated chemiluminescence of luminol on wireless conducting polymer films

Elena Villani et al.

Summary: In this study, electrochemiluminescence (ECL) was successfully generated from entirely pure conducting polymer films in alkaline aqueous solution, using PEDOT and PPy films prepared by constant current electrolysis as bipolar electrodes. ECL intensity profiles showed gradient emission on both materials, with different light-emitting regions. Imaging analysis allowed evaluation of ECL behavior of both CPs as a function of applied voltage and conductivity, showing potential applications in ECL and bipolar electrochemistry fields.

ELECTROCHIMICA ACTA (2021)

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Article Biochemical Research Methods

3D in vitro culture system to study collective migration in mammary organoid epithelium

Yunzhe Lu et al.

Summary: A new in vitro culture system for mammary gland organoid directional migration in response to FGF10 concentration gradient has been established, with a step-by-step protocol provided for preparing organoids and setting up the 3D culture system. The main technical difficulties in conducting the 3D migration assay involve selecting appropriate sizes of epithelial organoids and manually pairing organoids and FGF10-soaked beads within a desired distance. More detailed information can be found in Lu et al. (2020).

STAR PROTOCOLS (2021)

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Article Chemistry, Multidisciplinary

Imaging Cell-Matrix Adhesions and Collective Migration of Living Cells by Electrochemiluminescence Microscopy

Hao Ding et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

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Article Chemistry, Multidisciplinary

Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates

Kosuke Ino et al.

ACS SENSORS (2020)

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Article Chemistry, Analytical

Electrochemiluminescence (ECL)-Based Electrochemical Imaging Using a Massive Array of Bipolar Ultramicroelectrodes

Todd J. Anderson et al.

ANALYTICAL CHEMISTRY (2020)

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Article Electrochemistry

Electrochemical measurement of respiratory activity for evaluation of fibroblast spheroids containing endothelial cell networks

Kaoru Hiramoto et al.

ELECTROCHIMICA ACTA (2020)

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Review Chemistry, Multidisciplinary

Electrochemiluminescence Single-Cell Analysis: Intensity- and Imaging-Based Methods

Hao Ding et al.

CHEMPLUSCHEM (2020)

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Review Chemistry, Physical

Recent advances of scanning electrochemical microscopy and scanning ion conductance microscopy for single-cell analysis

Junjie Zhang et al.

CURRENT OPINION IN ELECTROCHEMISTRY (2020)

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Article Chemistry, Analytical

Fabrication of High-Density and Superuniform Gold Nanoelectrode Arrays for Electrochemical Fluorescence Imaging

Xiang Qin et al.

ANALYTICAL CHEMISTRY (2020)

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Article Cell Biology

Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis

Daniel Ruediger et al.

CELL REPORTS (2020)

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Article Chemistry, Multidisciplinary

Nanocage-confined electrochemiluminescence for the detection of dopamine released from living cells

Hao Ding et al.

CHEMICAL COMMUNICATIONS (2020)

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Article Chemistry, Multidisciplinary

Electrochemiluminescence-Based Capacitance Microscopy for Label-Free Imaging of Antigens on the Cellular Plasma Membrane

Jingjing Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

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Article Chemistry, Analytical

Steady-State Electrochemiluminescence at Single Senniconductive Titanium Dioxide Nanoparticles for Local Sensing of Single Cells

Chen Cui et al.

ANALYTICAL CHEMISTRY (2019)

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Review Chemistry, Multidisciplinary

Electrochemical imaging of cells and tissues

Tzu-En Lin et al.

CHEMICAL SCIENCE (2018)

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Article Chemistry, Multidisciplinary

Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis

Giovanni Valenti et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

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Review Chemistry, Analytical

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Kosuke Ino et al.

ANALYST (2017)

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Article Chemistry, Analytical

Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays

Yusuke Kanno et al.

ANALYTICAL CHEMISTRY (2017)

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Review Chemistry, Physical

Bioelectrochemical applications of microelectrode arrays in cell analysis and engineering

Kosuke Ino et al.

CURRENT OPINION IN ELECTROCHEMISTRY (2017)

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Article Electrochemistry

Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

Hiroya Abe et al.

ELECTROCHEMISTRY (2016)

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Article Chemistry, Analytical

Electrogenerated chemiluminescence of tris(2,2′ bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte

Noah Kebede et al.

ANALYST (2015)

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Article Chemistry, Analytical

Improving the Electrochemical Imaging Sensitivity of Scanning Electrochemical Microscopy-Scanning Ion Conductance Microscopy by Using Electrochemical Pt Deposition

Mustafa Sen et al.

ANALYTICAL CHEMISTRY (2015)

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Article Chemistry, Analytical

Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes

Kosuke Ino et al.

ANALYTICAL CHEMISTRY (2014)

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Article Chemistry, Multidisciplinary

Fluorescence Coupling for Direct Imaging of Electrocatalytic Heterogeneity

Joshua P. Guerrette et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

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Article Chemistry, Multidisciplinary

Environmentally friendly and highly sensitive Ruthenium(II) tris(2,2 '-bipyridyl) electrochemiluminescent system using 2-(dibutylamino) ethanol as co-reactant

Xiaoqing Liu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2007)

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Article Engineering, Chemical

Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering

Kosuke Ino et al.

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN (2007)

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Article Chemistry, Physical

Emission of tris(2,2′-bipyridine)ruthenium(II) by coreactant electrogenerated chemiluminescence:: From O2-insensitive to highly O2-sensitive

HZ Zheng et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2005)

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Article Chemistry, Multidisciplinary

Electrogenerated chemiluminescence 69:: The tris(2,2′-bipyridine)ruthenium(II), (Ru(bpy)32+)/tri-n-propylamine (TPrA) system revisited -: A new route involving TPrA•+ cation radicals

WJ Miao et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2002)

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Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds

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