Journal
DYES AND PIGMENTS
Volume 199, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.110058
Keywords
Water-soluble probe; Rapidly; Discriminate Cys; Hcy and GSH; Imaging; Dual fluorescence channels
Funding
- Natural Science Foundation of Shandong Province [ZR2019MB009, ZR2020MB009]
- National Natural Science Foundation of China [21672046, 21372054]
- Fund from the Huancui District of Weihai City
Ask authors/readers for more resources
A simple water-soluble probe was developed for rapid discrimination of different biothiols by dual excitation and emission wavelengths. The probe showed high selectivity and low detection limit, and was successfully used for differentiation imaging of biothiols in cells and zebrafish through dual fluorescent channels.
Biothiols are a class of amino acids containing mercaptan (thiol, SH) groups that have similar structures but play different roles in physiological activities, making it important to effectively distinguish them. Here, a simple water-soluble probe, PMCI, was developed for the first time to rapidly discriminate Cys/Hcy and GSH by dual excitation and emission wavelengths. The introduction of an N-Me pyridinium salt thioether group into the fluorophore structure not only makes the probe highly water-soluble, but also serves as a potential reactive site for biothiols, which together with an aldehyde group allows the simultaneous differential detection of Cys/Hcy and GSH. The probe itself has no background fluorescence due to the intramolecular photoinduced electron transfer (PET) effect. After reaction with biothiols, the probe solution showed different strong fluorescent signals (green: Cys and Hcy, red: GSH). It can respond quickly to biothiols (Cys: 190 s; Hcy: 155 s; GSH: 80 s) with high selectivity and low detection limit. Moreover, the probe has been successfully used for the rapid discrimination imaging of endogenous and exogenous biothiols in cells and zebrafish through dual fluorescent channels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available