Phenothiazine-based fluorescent probe for fluoride ions and its applications in rapid detection of endemic disease

Fluoride detection is important in environmental monitoring and disease screening. In many of detection methods, small molecule fluorescent probes have been developed rapidly in recent years for their high efficiency, selectivity and low cost. To develop quickly fluorescent probe for detection fluoride ions, a novel phenothiazinebased fluorescent probe PHT was designed and synthesized. The response time of PHT was only 1 min and the detection line of PHT reached to 1.3 ppb. Moreover, the stoke shift of PHT was close to 140 nm, which was much better than the previous reports. At last, the probe PHT was successfully used for HepG2 imaging and detection of biological samples with endemic disease.

Automated summary

Fluoride detection is crucial for environmental monitoring and disease screening. Small molecule fluorescent probes have gained rapid development in recent years due to their high efficiency, selectivity, and low cost. In this study, a novel phenothiazine-based fluorescent probe PHT was designed and synthesized for the quick detection of fluoride ions. PHT exhibited a response time of only 1 minute and a detection limit of 1.3 ppb. Furthermore, PHT showed a stoke shift of around 140 nm, which surpassed previous reports. Finally, the PHT probe was successfully utilized for HepG2 imaging and detection of biological samples with endemic disease.