Epithelial cells adapt to curvature induction via transient active osmotic swelling

Generation of tissue curvature is essential to morphogenesis. However, how cells adapt to changing curvature is still unknown because tools to dynamically control curvature in vitro are lacking. Here, we developed self-rolling substrates to study how flat epithelial cell monolayers adapt to a rapid anisotropic change of curvature. We show that the primary response is an active and transient osmotic swelling of cells. This cell volume increase is not observed on inducible wrinkled substrates, where concave and convex regions alternate each other over short distances; and this finding identifies swelling as a collective response to changes of curvature with a persistent sign over large distances. It is triggered by a drop in membrane tension and actin depolymerization, which is perceived by cells as a hypertonic shock. Osmotic swelling restores tension while actin reorganizes, probably to comply with curvature. Thus, epithelia are unique materials that transiently and actively swell while adapting to large curvature induction.

Automated summary

This study developed self-rolling substrates to investigate the adaptation of flat epithelial cell monolayers to rapid anisotropic changes in curvature. The primary response was found to be an active and transient osmotic swelling of cells, triggered by a drop in membrane tension and actin depolymerization. These findings suggest that epithelial cells are unique in their ability to transiently and actively swell while adapting to changes in curvature.