Journal
DEVELOPMENTAL CELL
Volume 57, Issue 8, Pages 1053-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2022.03.011
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Funding
- National Institutes of Health [R01AR076420, R01GM131642, P50CA121974, UM1DA051410]
- American Cancer Society [RSG-17-165-01-DDC]
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This study designed a computational approach to resolve signals and cell behaviors in organogenesis, revealing the role of Wnt/β-catenin and SHH signaling gradients in cell proliferation and spatial patterning.
Organ formation requires integrating signals to coordinate proliferation, specify cell fates, and shape tissue. Tracing these events and signals remains a challenge, as intermediate states across many critical transitions are unresolvable over real time and space. Here, we designed a unique computational approach to decompose a non-linear differentiation process into key components to resolve the signals and cell behaviors that drive a rapid transition, using the hair follicle dermal condensate as a model. Combining scRNA sequencing with genetic perturbation, we reveal that proliferative Dkk1+ progenitors transiently amplify to become quiescent dermal condensate cells by the mere spatiotemporal patterning of Wnt/beta-catenin and SHH signaling gradients. Together. they deterministically coordinate a rapid transition from proliferation to quiescence, cell fate specification, and morphogenesis. Moreover, genetically repatteming these gradients reproduces these events autonomously in slow motion across more intermediates that resolve the process. This analysis unravels two morphogen gradients that intersect to coordinate events of organogenesis.
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