Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 129, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2022.104349
Keywords
BAG3; Mitochondrial membrane potential; Apoptosis; White spot syndrome virus (WSSV); Scylla paramamosain
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Funding
- National Natural Science Foundation of China [42076125, 31802341]
- Natural Science Founda-tion of Guangdong Province, China [2018A030307044]
- Department of Education of Guangdong Province, China [2017KQNCX072]
- STUScientific Research Foundation for Talents [NTF18001]
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SpBAG3 protein was discovered in mud crab and found to play a crucial role in anti-apoptosis and antiviral immunity. Experimental results showed that SpBAG3 might promote WSSV infection by altering mitochondrial membrane potential.
The function of B-cell lymphoma-2 (Bcl-2) family proteins can be divided into two categories: anti-apoptotic and pro-apoptotic. As an anti-apoptotic protein, Bcl2-associated athanogene 3 (BAG3) plays a key role in regulating apoptosis, development, cell movement, and autophagy, and mediating the adaptability of cells to stimulation. However, SpBAG3 has not been reported in mud crab (Scylla paramamosain), and the regulatory effect of SpBAG3 on apoptosis in mud crab and its function in antiviral immunity is still unknown. In this study, SpBAG3 was found, and characterized, which encoded a total of 175 amino acid (molecular mass 19.3 kDa), including a specific conserved domain of the BAG family. SpBAG3 was significantly down-regulated at 0-48 h post-infection with WSSV in vivo. The antiviral effect of SpBAG3 was investigated using RNA interference. The results indicated that SpBAG3 might be involved in assisting the replication of WSSV in the host. SpBAG3 could change the mitochondrial membrane potential (o psi m), and affect cell apoptosis through mitochondrial apoptotic pathways. Therefore, the results of this study suggested that SpBAG3 could assist WSSV infection by inhibiting the apoptosis of the hemocytes in mud crab.
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