Journal
DEVELOPMENT
Volume 149, Issue 10, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200456
Keywords
Drosophila; Egg chamber; Basement membrane; Collagen; Laminin; Nidogen; Perlecan; Atomic force microscopy
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Funding
- Deutsche Forschungsgemeinschaft [DA586-16/2, EXC-2068-390729961]
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This study systematically analyzed the molecular composition and mechanical properties of the basement membrane underlying the follicle epithelium in Drosophila egg chambers. The results showed that Collagen IV played a major role in the stiffness of the basement membrane, while Perlecan and Collagen IV contributed greatly to the elongation and resistance against osmotic stress of the egg chambers.
The basement membrane is a specialized extracellular matrix (ECM) that is crucial for the development of epithelial tissues and organs. In Drosophila, the mechanical properties of the basement membrane play an important role in the proper elongation of the developing egg chamber; however, the molecular mechanisms contributing to basement membrane mechanical properties are not fully understood. Here, we systematically analyze the contributions of individual ECM components towards the molecular composition and mechanical properties of the basement membrane underlying the follicle epithelium of Drosophila egg chambers. We find that the Laminin and Collagen IV networks largely persist in the absence of the other components. Moreover, we show that Perlecan and Collagen IV, but not Laminin or Nidogen, contribute greatly towards egg chamber elongation. Similarly, Perlecan and Collagen, but not Laminin or Nidogen, contribute towards the resistance of egg chambers against osmotic stress. Finally, using atomic force microscopy we show that basement membrane stiffness mainly depends on Collagen IV. Our analysis reveals how single ECM components contribute to the mechanical properties of the basement membrane controlling tissue and organ shape.
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