4.7 Article

A Drosophila toolkit for HA-tagged proteins unveils a block in autophagy flux in the last instar larval fat body

Related references

Note: Only part of the references are listed.
Article Developmental Biology

Proximity-dependent labeling methods for proteomic profiling in living cells: An update

Justin A. Bosch et al.

Summary: Proteome composition of organelles and subcellular regions can be analyzed using proximity labeling-based methods coupled with mass spectrometry (MS) to understand cellular organization and protein interactome networks. These methods utilize enzymes that generate reactive radicals to covalently tag neighboring proteins, allowing for systematic analysis of spatially restricted proteomes. By fusing enzymes to specific proteins or signal peptides targeting to particular subcellular regions, spatial expression can be achieved for studying protein-protein interactions or identifying components in discrete subcellular compartments.

WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY (2021)

Article Multidisciplinary Sciences

p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response

Shun Kageyama et al.

Summary: Autophagy selectively degrades liquid droplets like p62/SQSTM1-bodies, influencing both autophagosome formation and Nrf2 activation. The findings suggest that p62-gels are not only substrates for autophagy, but also play a crucial role in antioxidative stress.

NATURE COMMUNICATIONS (2021)

Review Cell Biology

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Daniel J. Klionsky et al.

Summary: The guidelines presented in this article aim to assist researchers in selecting and interpreting methods to examine autophagy, as well as to help reviewers provide constructive critiques of reports focused on these processes. It emphasizes that no single assay is perfect for every situation, and multiple techniques may be needed to properly monitor autophagy in different experimental settings. The article also highlights the importance of targeting multiple autophagy-related genes in genetic approaches to block autophagy effectively.

AUTOPHAGY (2021)

Review Biochemistry & Molecular Biology

Regulation and function of V-ATPases in physiology and disease

Michael P. Collins et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Review Pharmacology & Pharmacy

Chloroquine against malaria, cancers and viral diseases

Wenmin Zhou et al.

DRUG DISCOVERY TODAY (2020)

Review Biotechnology & Applied Microbiology

Pick a Tag and Explore the Functions of Your Pet Protein

Giel Vandemoortele et al.

TRENDS IN BIOTECHNOLOGY (2019)

Review Cell Biology

Diverse Cellular Roles of Autophagy

Hideaki Morishita et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 35 (2019)

Article Multidisciplinary Sciences

A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

Ning Zhao et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy

Katharina N. Richter et al.

EMBO JOURNAL (2018)

Article Biochemical Research Methods

Salmon provides fast and bias-aware quantification of transcript expression

Rob Patro et al.

NATURE METHODS (2017)

Article Cell Biology

Drosophila Mitf regulates the V-ATPase and the lysosomal-autophagic pathway

Valentina Bouche et al.

AUTOPHAGY (2016)

Article Cell Biology

The Ccz1-Mon1-Rab7 module and Rab5 control distinct steps of autophagy

Krisztina Hegedus et al.

MOLECULAR BIOLOGY OF THE CELL (2016)

Article Biochemical Research Methods

Data-driven hypothesis weighting increases detection power in genome-scale multiple testing

Nikolaos Ignatiadis et al.

NATURE METHODS (2016)

Article Multidisciplinary Sciences

Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

Conrad M. Tenenbaum et al.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2016)

Article Biochemical Research Methods

Improved and expanded Q-system reagents for genetic manipulations

Olena Riabinina et al.

NATURE METHODS (2015)

Article Cell Biology

iLIR A web resource for prediction of Atg8-family interacting proteins

Ioanna Kalvari et al.

AUTOPHAGY (2014)

Article Biotechnology & Applied Microbiology

Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

Michael I. Love et al.

GENOME BIOLOGY (2014)

Article Developmental Biology

A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila

Johannes Bischof et al.

DEVELOPMENT (2013)

Article Endocrinology & Metabolism

Glucosylceramide modulates endolysosomal pH in Gaucher disease

Dan J. Sillence

MOLECULAR GENETICS AND METABOLISM (2013)

Review Physiology

Lysosomal Acidification Mechanisms

Joseph A. Mindell

ANNUAL REVIEW OF PHYSIOLOGY, VOL 74 (2012)

Article Biochemistry & Molecular Biology

Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila

Deborah L. Berry et al.