Alginate-based Composite Microspheres: Preparations and Applications for Bone Tissue Engineering

Alginate-based biomaterials have been extensively studied for bone tissue engineering. Scaffolds, microspheres, and hydrogels can be developed using alginate, which is biocompatible, biodegradable, and able to deliver growth factors and drugs. Alginate microspheres can be produced using crosslinking, microfluidic, three-dimensional printing, extrusion, and emulsion methods. The sizes of the alginate microspheres range from 10 mu m to 4 mm. This review describes the chemical characterization and mechanical assessment of alginate-based microspheres. Combinations of alginate with hydroxyapatite, chitosan, collagen, polylactic acid, polycaprolactone, and bioglass were discussed for bone tissue repair and regeneration. In addition, alginate combinations with bone morphogenetic proteins, vascular endothelial growth factor, transforming growth factor beta-3, other growth factors, cells, proteins, drugs, and osteoinductive drugs were analyzed for tissue engineering applications. Furthermore, the biocompatibility of developed alginate microspheres was discussed for different cell lines. Finally, alginate microsphere-based composites with stem cell interaction for bone tissue regeneration were presented. In the present review, we have assessed the preclinical research on in vivo models of alginate-based microspheres for bone tissue repair and regeneration. Overall, alginate-based microspheres are potential candidates for graft substitutes and the treatment of various bone-related diseases.

Automated summary

Alginate-based microspheres are important materials for studying bone tissue engineering. They are biocompatible and biodegradable, and can be used to fabricate scaffolds, microspheres, and hydrogels that can deliver growth factors and drugs. Alginate microspheres can be produced in various sizes and combined with other substances such as hydroxyapatite and collagen for bone tissue repair and regeneration. In addition, alginate microspheres can interact with cells, proteins, drugs, and stem cells, making them versatile in tissue engineering applications.