Effects of polyvinyl chloride microplastics on reproduction, oxidative stress and reproduction and detoxification-related genes in Daphnia magna

The drastic increase of microplastics (MPs) in aquatic environment has become a serious threat to marine and freshwater ecosystems. However, little information is available regarding the potential detrimental effects of polyvinyl chloride microplastics (PVC-MPs) on aquatic organisms. This study investigated the changes of reproduction parameters, oxidative stress and the expression of reproduction and detoxification-related genes in Daphnia magna after exposed to 2 & PLUSMN; 1 and 50 & PLUSMN; 10 mu m PVC-MPs. The results showed that chronic exposure to 2 & PLUSMN; 1 mu m PVC-MPs prolonged days to the first brood, increased total number of broods per female and frequency of molting per adult, decreased offspring number at first brood and total number of offspring per female in D. magna. Moreover, 2 & PLUSMN; 1 mu m PVC-MPs also disturbed the activities of SOD and CAT, increased GSH and MDA levels. The expression of Vtg, SOD, CAT, CYP314 and CYP360A8 genes also exhibited different response patterns depending on exposure time. Furthermore, 50 & PLUSMN; 10 mu m PVC-MPs decreased offspring at first brood and Vtg mRNA level, increased the transcription levels and activities of SOD and CAT. These results suggest that the presence of PVC-MPs in aquatic environment may cause reproduction toxicity by disrupting the reproduction and detoxification-related genes expression and inducing oxidative stress in D. magna.

Automated summary

The drastic increase of microplastics in aquatic environment is a serious threat to marine and freshwater ecosystems. This study investigated the potential harmful effects of polyvinyl chloride microplastics on aquatic organisms. The results showed that chronic exposure to PVC-MPs affected reproduction parameters, oxidative stress, and gene expression in Daphnia magna, suggesting that PVC-MPs in aquatic environment may cause reproduction toxicity.