Achieving reinforced broad-spectrum and sustained antimicrobial efficacy by nickel-doping AlOOH nanoflower accommodated with uniform silver nanospecies

Two-dimensional materials composed of all-oriented branches assembled with active Ag nanospecies might be attractive in the microbial inhibition application because of improved Ag ion release behavior and interaction with targeted substance. Herein, we report an excellent Ag-AlNiO nanocomposite antimicrobial materials by Nidoping AlOOH triggering better implanting of Ag nanospecies. The TEM results revealed that the formed nanoflower-like configuration of the Ni-doping AlOOH favors the formation of refined active silver nanospecies (diameter of 6-10 nm) compared to Ni-free AlOOH (Ag-AlO). The resulting Ag-AlNiO afford good biocompatibility which is evidenced by the cell viability test via cell counting kit-8 (CCK-8) assay. More importantly, Ag AlNiO showed promotive broad-spectrum antimicrobial properties against various gram-negative bacteria, gram positive bacteria, and fungi. We also demonstrated that corresponding MIC values against different microbes ranging from 16 to 128 mu g/mL concerning Ag-AlNiO composite were at least 2-, or 4-folds lower than Ag-AlO composite. Besides, the optimal Ag-AlNiO is endowed with excellent antimicrobial effect against H37Rv, Bacillus Calmette-Guerin (BCG), and multidrug-resistant (MDR) strain of Mycobacterium tuberculosis. In comparison with Ag-AlO, Ag-AlNiO revealed a prolonged inhibitory effect on microbial growth, which indicated that abundant silver nanospecies were sustained released from the nanoflower-like Ag-AlNiO composites in addition to the direct attacks.

Automated summary

In this study, an excellent Ag-AlNiO nanocomposite antimicrobial material was reported by Nidoping AlOOH triggering better implanting of Ag nanospecies. The nanoflower-like configuration of the Ni-doping AlOOH favors the formation of refined active silver nanospecies. Ag-AlNiO shows good biocompatibility and broad-spectrum antimicrobial properties against various gram-negative bacteria, gram positive bacteria, and fungi. Compared to Ag-AlO, Ag-AlNiO has a prolonged inhibitory effect on microbial growth.