4.6 Article

In-situ growth of multienzyme-inorganic hybrid nanoflowers on PVA-co-PE nanofibrous strip for colorimetric biosensor

Publisher

ELSEVIER
DOI: 10.1016/j.colsurfa.2022.128419

Keywords

Nanofiber; Hybrid nanoflower; Visual detection; Glucose sensor

Funding

  1. National Natural Science Foundation of China [51873165, U20A20257]
  2. Science and Technology Innovation Major Projects of Hubei Prov-ince [2019AAA035]
  3. Key Research and Development Program of Shandong Province of China [2019JZZY010338]
  4. Guangdong Basic and Applied Basic Research Foundation [2019A1515110432]
  5. Hubei International Scientific and Technological Coopera-tion Base of Intelligent Textile Materials Application
  6. Wuhan Engineering Technology Research Center for Advanced Fibers

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A hierarchically porous visual colorimetric nanofiber-based strip was developed for highly sensitive detection of glucose. The strip showed high sensitivity to glucose and excellent specificity, making it a promising candidate for personal diabetes detection.
With the increasing demand of health monitoring and protection, Point-of-Care Testing has developed rapidly due to its convenience and high efficiency. Here, a visual colorimetric nanofiber-based strip with a hierarchically porous structure was prepared for highly sensitive detection of glucose. To facilitate the subsequent testing, the composites with a hybrid nanoflower structure containing glucose oxidase (GOx), horseradish peroxidase (HRP), 3,3 & PRIME;5,5 & PRIME;-tetramethylbenzidine (TMB) and Mn-3(PO4)(2) were immobilized on a hierarchically porous PVA-co-PE nanofibrous strip. The obtained strip was used to detect glucose, a strong linear correlation (R-2 = 0.996) was found between the color difference and ln(C-glucose) in the range of 0.25-10 mM glucose. The detection limit is 0.14 mM and a discernible color change could be detected with the naked eye at low glucose concentration, indicating that the strip is highly sensitive to glucose. Additionally, a series of interferences substances including NaCl, KCl and urea were also investigated, and the results showed excellent specificity to glucose. Moreover, the strip exhibits good stability in the pH range of 5-7 and maintains excellent glucose detection performance after 60 days. It demonstrates that this strip is a promising candidate for personal diabetes detection.

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