4.7 Article

Bioaugmentation with methanogenic culture to improve methane production from chicken manure in batch anaerobic digestion

Journal

CHEMOSPHERE
Volume 303, Issue -, Pages -

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.135127

Keywords

Chicken manure; Anaerobic digestion; Bioaugmentation efficiency; Methanothrix

Funding

  1. National Nature Science Foundation of China, Beijing, China [52170143]
  2. Strategic Priority Research Pro-gram of the Chinese Academy of Sciences, Beijing, China [XDA21050400]
  3. Beijing, China
  4. Guangzhou Science and Technology project, Guangzhou, China [2021A1515012082]
  5. Youth Innovation Promotion Association, CAS, Beijing, China

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The study showed that bioaugmentation increased methane yield and shortened methane production time in batch reactors. Higher bioaugmentation doses did not significantly improve efficiency, with a recommended dosage of 0.27 g VSBS/g VSCM. Additionally, whole genome pyrosequencing revealed changes in the dominant methanogenic pathways with bioaugmentation, indicating enhanced metabolic activities in the digestor.
This study sought to investigate the effect of bioaugmentation on batch anaerobic digestion of chicken manure. The digestion performance with and without bioaugmentation and bioaugmented efficiency under different dosages were compared. The results demonstrated that bioaugmentation increased the methane yield and shortened the methane production time in batch reactors. Compared to the un-bioaugmented control, the methane yield of bioaugmented digesters was increased by 1.2-, 1.7-, 2.2-, 3.4-, and 3.6-fold at addition ratios of 0.07, 0.14, 0.21, 0.27, and 0.34 g VS bioaugmentation seed (BS)/g VSCM, respectively. However, higher bioaugmentation doses (0.34 g VSBS/g VSCM) did not exhibit significantly improved bioaugmentation efficiency, thus, the recommended dose is 0.27 g VSBS/g VSCM for biomethane conversion of CM. Moreover, whole genome pyrosequencing revealed that Methanoculleus and Methanobrevibacter predominated the non-bioaugmentation digesters, whereas Methanothrix, Methanobacterium, and Methanomassiliicoccus were the dominant methanogens in bioaugmentation digesters. The increased methane may be explained by an increase in the Methanothrix population, which accelerated acetic acid degradation. With bioaugmentation the mainly methanogenic pathways have become more diverse. From gene function perspective, bioaugmentation enhanced metabolic activities in digestor which function better in metabolism.

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