The effects of biochar aging on rhizosphere microbial communities in cadmium-contaminated acid soil

Biochars are widely used in the remediation of Cd-contaminated soils. However, changes in the bacterial communities in the rhizosphere contaminated with Cd in response to biochar aging are poorly studied. Addressing this gap in knowledge is important to improving micro-ecological services on healthy growth of plants with mitigation strategies against Cd contamination. An aging experiment (270 days) was conducted with biochars derived from poultry litter and sugar-gum wood added to a Cd-contaminated acid soil. Bacterial communities in the rhizosphere of Brassica rapa and bulk soils were investigated after 1, 90 and 270 days of biochar aging. There was no significant difference (P > 0.05) in bacterial Shannon and Simpson indices between the control and biochar treatments. However, compared to the no-Cd control, the addition of Cd decreased the relative abundances of Firmicutes, Chloroflexi and Acidobacteriota but increased those of Actinobacteriota and Proteobacteria. Poultry-litter biochar had the largest effect on bacterial community composition, especially in the rhizosphere. Aging of poultry-litter biochar increased the abundance of Armatimonadota over time more than the sugar-gum-wood biochar, which was attributed to a lower pH and higher bioavailability of Cd in the sugar-gum-wood biochar treatment. The addition of poultry-litter biochar to the contaminated soil mitigated the bioaccumulation of Cd by increasing soil pH and restoring soil bacterial ecology in contaminated acid soils over time.

Automated summary

This study investigated the impact of biochar aging on bacterial communities in Cd-contaminated rhizosphere, and found that poultry-litter biochar had the greatest effect on bacterial community composition. The addition of poultry-litter biochar mitigated the bioaccumulation of Cd in contaminated soils by increasing soil pH and restoring soil bacterial ecology over time.