Role of cytochrome P450 genes of Trichoderma atroviride T23 on the resistance and degradation of dichlorvos

The Trichoderma has been extensively used to degrade the xenobiotics. In the present study, thirty-nine open reading frames of cytochrome P450 genes from T. atroviride T23 genome was cloned and it was found to be distributed in 29 families under 21 clades. Among them, 21 cytochrome P450 genes were involved in the degradation of xenobiotics. The quantitative expression of P450 genes in the presence of dichlorvos at 24 h showed 7 different expression patterns in the presence of 100 mu g/mL, 300 mu g/mL, 500 mu g/mL and 1000 mu g/mL of dichlorvos. The relative expression of P450 genes belongs to the family of TaCyp548, TaCyp620, TaCyp52, TaCyp528, TaCyp504 were upregulated at least 1-fold compared to the control. Significantly, the deletion of TaCyp548-2 reduced the concentration of 2,2-dichloroethanol. Further, it was observed that TaCyp548-2 belongs to the o-hydroxylase family was responsible for fatty acid oxidation and the production of acetic acid, propionic acid, isobutyric acid and dibutyric acid to convert the 2,2-dichloroethanol to 2,2-dichloroethanolacetate. This study evidenced the involvement of Trichoderma P450 genes on dichlorvos degradation as an environmentally significant Biological control agent for the sustainable agriculture.

Automated summary

This study cloned thirty-nine cytochrome P450 genes from the T. atroviride T23 genome, with 21 of them being involved in xenobiotic degradation. The quantitative expression of these genes showed different patterns in the presence of dichlorvos at varying concentrations. Specifically, the deletion of TaCyp548-2 reduced the concentration of 2,2-dichloroethanol, indicating its role in the degradation process.