Acceleration of biotic decolorization and partial mineralization of methyl orange by a photo-assisted n-type semiconductor

In this study, a n-type semiconductor perylene diimide (PDI) was coupled with biodegradation to accelerate the biotic decolorization and mineralization of methyl orange (MO) under light condition. The decolorization rates (k(1)) of MO in pure and mixed cultures with PDI were promoted by 1.35 and 1.79 folds, respectively, comparing to the cultures without PDI. The total mineralization efficiency of 4-aminobenzenesulfonic acid (4-ABA) was achieved to 22.10 +/- 0.84% when in the presence of PDI. The quinone-like group and oxidation-reduction ca-pacity of PDI were detected by Fourier transform infrared spectroscopy and cyclic voltammetry, respectively, but the enhancement on the biotic decolorization of MO was not promoted under dark condition indicating that microbial extracellular electron transfer was not promoted. The 4-ABA was confirmed to be partially mineralized when the PDI exposure to light. The generated free radicals i.e., h(+), center dot OH, was demonstrated as active species to accelerate the decolorization and mineralization of MO by ESR test and radical quenching experiments. The bond breaking of MO and 4-ABA molecules were successfully predicted by density functional theory calculations and were further proven by liquid-chromatography mass spectra. The synergistic mechanism of decolorization and mineralization of MO by microorganism and photocatalysis was proposed. Moreover, High-throughput sequencing and Live/dead cell results indicated that the presence of PDI has no obvious toxicity to the micro -organisms and will not change the microbial communities during the short-term treatment period. The results of study provided a biological intimate photocatalytic material and suggested a feasible way for its combination with biodegradation of azo dyes.

Automated summary

In this study, coupling n-type semiconductor perylene diimide (PDI) with biodegradation was found to significantly enhance the biotic decolorization and mineralization of methyl orange, with the generation of free radicals identified as a key factor in accelerating the process. The study provides insights into the synergistic mechanism of microbial and photocatalytic action, offering a feasible approach for the combination of biodegradation and photocatalysis for azo dyes.