Journal
CANCER LETTERS
Volume 529, Issue -, Pages 112-125Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2022.01.004
Keywords
Colorectal cancer; Monocytes; CXCR2; Interferon; Tumour-associated macrophages
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Funding
- National Natural Science Foundation of China [81702815, 81972005]
- Key Technology Research and Development Program of Shandong Province [2019GSF108247]
- Natural Science Foundation of Shandong Province [ZR2020LZL015, ZR2017MH044]
- Science and Technology Development Project in Jinan [201805084]
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This study found that CXCR2 expression decreases in circulating monocytes of patients with colorectal cancer (CRC) and is negatively associated with systemic inflammatory markers and positively associated with intratumoural immunocyte infiltration. The pro-inflammatory cytokine IFN-gamma downregulates CXCR2 expression of monocytes/TAMs by promoting GRK-2 expression. The CXCR2 signaling pathway is important for the chemotaxis of monocytes and their responsiveness to LPS stimulation. Monocytes with decreased CXCR2 expression show distinct phenotypes and functions after differentiating into TAMs.
Though circulating monocytes are the main source of tumour-associated macrophages (TAMs), the regulatory mechanisms of their recruitment to tumours and further differentiation remain unclear. In the present study, we observed a significant decrease in CXCR2 expression in classical circulating monocytes of patients with colorectal cancer (CRC), particularly those in the late TNM stage. The percentage of CXCR2+ monocytes was negatively associated with systemic inflammatory markers and positively associated with intratumoural immunocyte infiltration. The pro-inflammatory cytokine IFN-gamma, which was overexpressed in patients with CRC, downregulated CXCR2 expression of monocytes/TAMs by promoting GRK-2 expression. In vitro, inhibition of CXCR2 signalling in monocytes led to impaired chemotaxis to the tumour cell line supernatant and lower responsiveness to lipopolysaccharide (LPS) stimulation. Finally, monocytes from patients with CRC with decreased CXCR2 expression showed distinct phenotypes and functions after differentiating into CRC cell lineeducated TAMs, including expression of co-stimulatory factors and secretion profile, than those from healthy controls. GRK-2 inhibitor altered the functional characteristics of TAMs. In summary, our findings suggest that CXCR2 expression on circulating monocytes reflects CRC stages and is an important factor determining TAM composition in the tumour microenvironment.
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