Development of a droplet digital PCR assay to detect bovine alphaherpesvirus 1 in bovine semen

Article Infectious Diseases

Digital droplet PCR for the detection and quantification of circulating bovineDeltapapillomavirus

Francesca De Falco et al.

Summary: This study compared the levels of BPV DNA in bovine samples using ddPCR and qPCR, finding that ddPCR was more sensitive and accurate than qPCR, allowing the detection of more BPV genotypes.

TRANSBOUNDARY AND EMERGING DISEASES (2021)

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Article Biotechnology & Applied Microbiology

Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets

Changwoo Park et al.

Summary: The study found that ddPCR is more sensitive than RT-qPCR in detecting viral RNA, especially for determining the copy number of reference materials.

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2021)

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Review Medical Laboratory Technology

Droplet digital PCR as an emerging tool in detecting pathogens nucleic acids in infectious diseases

Biao Chen et al.

Summary: The microbial infectious diseases pose a major global public health challenge, with ddPCR emerging as a promising tool for detecting pathogenic microorganisms. The ddPCR method offers high sensitivity, precision, and absolute quantification in detecting targeted DNA of infectious microorganisms.

CLINICA CHIMICA ACTA (2021)

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Review Virology

Droplet digital PCR of viral left-to-right markDNA/RNA, current progress, challenges, and future perspectives

Amir Asri Kojabad et al.

Summary: ddPCR is a high-throughput digital PCR method that offers high sensitivity, specificity, absolute quantification without the need for a standard curve, good reproducibility, and tolerance to PCR inhibitors, making it a valuable addition to virologists' toolbox. Recent technological advances in ddPCR methods and their applications in viral identification are outlined in this review.

JOURNAL OF MEDICAL VIROLOGY (2021)

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Article Virology

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)

Samuel Long et al.

Summary: This study describes the design and testing of a sensitive SIV reverse transcription droplet digital PCR assay based on the Raindance ddPCR platform. By exploring different priming conditions and reverse transcriptases, the background signals were eliminated to enable the detection and quantification of low-level target signals. Further development of similar assays for sensitive detection of low-level targets in RNA samples can be explored.

VIROLOGY JOURNAL (2021)

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Article Immunology

Field Evaluation of Commercial Vaccines against Infectious Bovine Rhinotracheitis (Ibr) Virus Using Different Immunization Protocols

Laureana De Brun et al.

Summary: The study evaluated the performance of four commercially available BoHV-1 vaccines, with only vaccine A inducing significant levels of neutralizing antibodies. The combination of vaccine D with other vaccines did not affect the neutralizing antibody responses to BoHV-1.

VACCINES (2021)

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Review Veterinary Sciences

Review of Infections With Bovine Herpesvirus 1 in Slovenia

Peter Hostnik et al.

Summary: In the 1950s, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) disease was first clinically detected and documented in cattle in Slovenia. The bovine herpes virus 1 (BoHV-1) was confirmed from infected herds by virus isolation on cell cultures. High biosecurity measures were introduced to keep the breeding centers virus-free.

FRONTIERS IN VETERINARY SCIENCE (2021)

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Article Agriculture, Dairy & Animal Science