Journal
BMC PLANT BIOLOGY
Volume 22, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12870-022-03618-5
Keywords
Abiotic stress; GRAS transcription factor; Expression analysis; Molecular characterization; Sweetpotato
Categories
Funding
- National Natural Science Foundation of China [32171936]
- Xuzhou Science and Technology Planning Project [KC21117]
- Natural science fund for colleges and universities in Jiangsu Province [21KJB180014]
- China Agriculture Research System of MOF and MARA [CARS-10-B02]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- postgraduate research & practice innovation program of Jiangsu province [KYCX20_2294, KYCX21_2590]
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This study provides a comprehensive survey on GRAS genes in sweetpotato and reveals their important roles in adverse environmental conditions, suggesting their potential as regulators for molecular breeding of tolerant sweetpotato.
Background The plant-specific GRAS transcription factors play pivotal roles in various adverse environmental conditions. Numerous GRAS genes have been explored and characterized in different plants, however, comprehensive survey on GRASs in sweetpotato is lagging. Results In this study, 72 putative sweetpotato IbGRAS genes with uneven distribution were isolated on 15 chromosomes and classified into 12 subfamilies supported by gene structures and motif compositions. Moreover, both tandem duplication and segmental duplication events played critical roles in the expansion of sweetpotato GRAS genes, and the collinearity between IbGRAS genes and the related orthologs from nine other plants further depicted evolutionary insights into GRAS gene family. RNA-seq analysis under salt stress and qRT-PCR detection of 12 selected IbGRAS genes demonstrated their significant and varying inductions under multiple abiotic stresses (salt, drought, heat and cold) and hormone treatments (ABA, ACC and JA). Consistently, the promoter regions of IbGRAS genes harbored a series of stress- and hormone-associated cis-acting elements. Among them, IbGRAS71, the potential candidate for breeding tolerant plants, was characterized as having transactivation activity in yeasts, while IbGRAS-2/-4/-9 did not. Moreover, a complex interaction relationship between IbGRASs was observed through the interaction network analysis and yeast two-hybrid assays. Conclusions Our results laid a foundation for further functional identifications of IbGRAS genes, and multiple members may serve as potential regulators for molecular breeding of tolerant sweetpotato.
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