Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 27, Issue 2, Pages 171-182Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-021-0248-1
Keywords
rosmarinic acid; reactive oxygen species; DNA damage; apoptosis; factor erythroid 2-related factor 2; heme oxygenase-1
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korea government [2017R1D1A1B03033263, 2021R1A2C200954911]
- National Research Foundation of Korea [2017R1D1A1B03033263] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study demonstrates that rosmarinic acid (RA) can protect C2C12 myoblasts from H2O2-induced damage by inhibiting apoptosis, reducing ROS production, preserving DNA integrity, maintaining mitochondrial function, and activating the Nrf2/HO-1 antioxidant pathway.
Rosmarinic acid (RA) is a naturally occurring polyphenolic compound that has been applied as a therapeutic antioxidant. However, its action mechanism in muscle cells remains unclear. The objective of the current study was to explore the protective effect of RA against DNA damage and apoptotic cell death caused by hydrogen peroxide (H2O2) in mouse C2C12 myoblast cells and clarify related signaling pathway. Our results showed that pretreatment with RA significantly improved cell viability, inhibited apoptosis, and suppressed the generation of reactive oxygen species (ROS) in H2O2-treated C2C12 cells. Additionally, DNA damage triggered by H2O2 was abrogated by RA pretreatment. Moreover, H2O2-induced mitochondrial dysfunctions associated with apoptotic events, including loss of mitochondrial membrane potential and adenosine triphosphate content, up-regulation of Bax, down-regulation of Bcl-2, and cytosolic release of cytochrome c, were reduced in the presence of RA. RA also attenuated H2O2-induced activation of caspase-9 and caspase-3, which was associated with the ability of RA to block the degradation of poly (ADP-ribose) polymerase. Furthermore, RA dramatically promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and its phosphorylation in the presence of H2O2 with concomitant upregulation of heme oxygenase-1 (HO-1) expression, a major downstream factor of Nrf2. Taken together, these results suggest that RA could protect C2C12 myoblasts from H2O2-induced damage by maintaining mitochondrial function while eliminating ROS along with activation of the Nrf2/HO-1 antioxidant pathway, indicating that RA may be a potential therapeutic candidate to treat oxidative stress-mediated injury.
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